HLF-a细胞
数量： 大量
年限： 54 years
ATCC Number： CCL-199™
运输方式： 冻存运输
相关**： 上皮细胞癌
是否是肿瘤细胞： 1
物种来源： 人
器官来源： 肺
生长状态： 贴壁生长
细胞形态： 成纤维样
HLF-a细胞Designations: HLF-a
Depositors: GD Stoner
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: lung
Disease: epidermoid carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. HLF-a细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: February, 1976
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 12,13
D5S818: 8,12
D7S820: 9,12
THO1: 6,9.3
TPOX: 6,8
vWA: 15
Cytogenetic Analysis: human female; diploid; stable
Isoenzymes: G6PD, B
Age: 54 years
HLF-a细胞Gender: female
Ethnicity: Black
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: HLF-a细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
