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NCI-H661细胞

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  • 产品名称:NCI-H661细胞
  • 产品型号:NCI-H661
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-08-08
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简单介绍
NCI-H661细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。NCI-H661细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

NCI-H661细胞

ATCC Number: HTB-183™

相关**: 其他**

运输方式: 冻存运输

年限: 43 years

细胞形态: 上皮样

是否是肿瘤细胞: 1

物种来源: 人

数量: 大量

生长状态: 贴壁生长

器官来源: 肺

Designations: NCI-H661 [H661]

Depositors: AF Gazdar, JD Minna

NCI-H661细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: lung

Disease: carcinoma; large cell lung cancer

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Bethesda Maryland,

Isolation date: 1982

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 11

D16S539: 12

NCI-H661细胞D5S818: 11

D7S820: 8,10

THO1: 8

TPOX: 8

vWA: 17

Cytogenetic Analysis: hyperhexaploid; modal number = 142; range = 130 to 153. Each cell had over 70 marker chromosomes. Among the markers were t(1p10q), del(1)(q11/q12), 6q+, 2q+,der(20)t(12;20)(q11;q13). Some of these had 2 to copies per cell. Most cells also had 5 to 10 microchromosomes (metacentric like). Structurally normal N2,N4 and N9 were absent; two copies of the X and multiple copies of the Y were detected.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 0

PGM1, 1

PGM3, 1

Age: 43 years

Gender: male

Ethnicity: Caucasian

Comments: The line lacks ultrastructural and biochemical evidence of squamous differentiation or mucin production.

The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities.

The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.

Propagation: NCI-H661细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA, rinse quickly and remove trypsin leaving 1 to 2 ml. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, disperse cells and transfer to a new flask.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

22437: Levitt ML, et al. NCI-H661细胞Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: 1967140

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