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SHP-77细胞

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  • 产品名称:SHP-77细胞
  • 产品型号:SHP-77
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-09-16
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简单介绍
SHP-77细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。SHP-77细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

SHP-77细胞

器官来源: 肺

运输方式: 冻存运输

数量: 大量

ATCC Number: CRL-2195™

相关**: 小细胞肺癌

年限: 54 years

细胞类型: 其他细胞类型

生长状态: 悬浮生长

细胞形态: 上皮样

是否是肿瘤细胞: 1

物种来源: 人

Designations: SHP-77

SHP-77细胞Depositors: AM Koros

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension and loosely adherent

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: lung

Disease: carcinoma; small cell lung cancer

Cell Type: large cell, variant;

Cellular Products: SHP-77细胞neural cell adhesion molecule (NCAM) NKH-1

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Antigen Expression: Blood Type O; Rh +; CD56; CD57 (HNK-1,Leu-7)

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 8

D16S539: 11

D5S818: 12

D7S820: 11

THO1: 7

TPOX: 9,11

vWA: 16

Age: 54 years

Gender: male

Ethnicity: Caucasian

Comments: SHP-77细胞The SHP-77 (Shadyside Hospital, Pittsburgh, PA) cell line is a biochemically stable continuously cultured cell line which has retained important features of SCLC.

It was established in 1977 by Edwin R. Fisher and John D. Paulson.

The line was derived from a non-encapsulated primary lung tumor from the apical portion of the upper lobe of the left lung.

This cell line is an unusual undifferentiated large cell variant of small cell lung carcinoma.

It has the morphology of a variant, but the biochemical properties of a classic SCLC.

Electron microscopy revealed the presence of gland formation and intracytoplasmic lamellar bodies.

The cells have neuroendocrine markers L-dopa decarboxylase and dense core secretory granules.

SHP-77 can serve as an in vitro target in 51Cr and 111In release cytotoxicity assays as well as in vivo nude mice assays for evaluating immune reactivity of cells and serum from lung cancer patients.

The cells can be used to evaluate the immune status of patients with SCLC who are treated with radiation or chemotherapy.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 3 to 4 days

Cultures can be maintained by addition or replacement of fresh medium.

Start cultures at 4 to 5 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

The cells grow as floating clusters with some loosely adherent cells, but only the floating cells are transferred.

Preservation: Culture medium, 95%; DMSO, 5%

Doubling Time: 96 hrs

Related Products: SHP-77细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

References: 21861: . Immune-deficient animals. Basel: Karger; 1984.

22751: . . J. Exp. Clin. Res. 4: 355-363, 1985.

22982: Fisher ER, et al. Comparative histopathologic, histochemical, electron microscopic and tissue culture studies of bronchial carcinoids and oat cell carcinomas of lung. Am. J. Clin. Pathol. 69: 165-172, 1978. PubMed: 204185

23055: Koros AM, et al. Stability and utility of the unique human small cell carcinoma line SHP-77. Cancer Res. 45: 2725-2731, 1985. PubMed: 2985251

23090: Carbone DP, et al. Neural cell adhesion molecule expression and messenger RNA splicing patterns in lung cancer cell lines are correlated with neuroendocrine phenotype and growth morphology. Cancer Res. 51: 6142-6149, 1991. PubMed: 1718595

23372: Fisher ER, Paulson JD. A new in vitro cell line established from human large cell variant of oat cell lung cancer. Cancer Res. 38: 3830-3835, 1978. PubMed: 212181

23375: Koros AM, et al. 186Rhenium-HNK1 monoclonal antibody targets human small cell lung cancer cells in athymic nude mice: rapid screening model for therapy. Anticancer Res. 13: 1953-1956, 1993. PubMed: 7507652

24247: Koros. Monoclonal antibodies directed against small cell carcinomas define by flow cytometry phenotypic differences among small cell and non-small cell carcinomas, neuroblastomas, and lymphoblastoid cell lines. Lung Cancer 4: 52-54, 1988.

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