Sar Nis细胞
数量： 大量
细胞形态： 成纤维样
ATCC Number： CRL-1231™
器官来源： 皮肤
相关**： 其他**
是否是肿瘤细胞： 1
物种来源： 人
年限： 22 years
生长状态： 贴壁生长
运输方式： 冻存运输
Sar Nis细胞Designations: Sar Nis
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: pseudoachondroplasia (autosomal dominant)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Sar Nis细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 22 years
Gender: female
Comments: The tissue donor suffered from pseudoachondroplasia (autosomal dominant form).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Sar Nis细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Sar Nis细胞Subculture every 6 to 8 days.
