182-PF SK细胞
ATCC Number： CRL-1532™
相关**： 其他**
运输方式： 冻存运输
数量： 大量
细胞形态： 成纤维样
是否是肿瘤细胞： 1
物种来源： 人
年限： 20 years
器官来源： 皮肤
182-PF SK细胞生长状态： 贴壁生长
Designations: 182-PF SK
Depositors: L Kopelovich
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: hereditary adenomatosis
Permits/Forms: 182-PF SK细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 20 years
Gender: male
Ethnicity: Caucasian
Comments: The tissue donor suffered from hereditary adenomatosis.
The line is capable of 10 population doublings after thawing.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: 182-PF SK细胞Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 days.
References: 22736: Rhim JS, et al. Chemical transformation of cultured skin fibroblasts from humans genetically predisposed to cancer. Cancer Detect. Prev. 4: 239-247, 1981. PubMed: 7349781
22759: . . Prog. 182-PF SK细胞Cancer Res. Ther. 13: 97-108, 1980.
