HuNS1细胞
数量： 大量
生长状态： 悬浮生长
ATCC Number： CRL-8644™
相关**： 其他**
细胞形态： **样
运输方式： 冻存运输
细胞类型： **母细胞
是否是肿瘤细胞： 1
物种来源： 人
Designations: HuNS1
Depositors: Medical College of Wisconsin Research Foundation, Inc.
HuNS1细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: myeloma
Cell Type: lymphoblast
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DNA Profile (STR): Amelogenin: X,Y
HuNS1细胞CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 10,12
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 16,19
Gender: male
Comments: HuNS1细胞This line was derived from the GM4672 cell line (Human Genetic Mutant Cell Repository, Coriell Institute for Medical Research) following treatment with ethylmethanesulfonate (EMS).
The cells are HAT sensitive, do not secrete immunoglobulin and are negative for Epstein-Barr virus.
The line is suitable for use as a fusion partner for human B cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 4.5g/L glucose, 10 mM HEPES, 1 mM sodium pyruvate, 1.5 g/L sodium bicarbonate, 0.02 mM 2-amino-6-methylmercaptopurine, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: Complete Culture Medium, 95%; DMSO, 5%.
Storage temperature: liquid nitrogen vapor phase
References: 3864: Winkelhake JL. HuNS1细胞Myelomas for producing human/human hybridomas. US Patent 4,720,459 dated Jan 19 1988
