Hs 895.T细胞
细胞形态： 成纤维样
是否是肿瘤细胞： 1
物种来源： 人
运输方式： 冻存运输
年限： 48 years
生长状态： 贴壁生长
数量： 大量
器官来源： 皮肤
ATCC Number： CRL-7637™
相关**： 黑色素瘤
Hs 895.T细胞Designations: Hs 895.T
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: melanoma
Derived from metastatic site: lung
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 895.T细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 12,14
D5S818: 11,12
D7S820: 10,12
THO1: 6,7
TPOX: 8,11
vWA: 18
Age: 48 years
Gender: female
Ethnicity: Caucasian
Comments: Hs 895.T细胞Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
This melanoma cell line was isolated from the same patient as Hs 895.Sk (ATCC CRL-7636), a normal skin fibroblast. Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Hs 895.T细胞recommended serum:ATCC 30-2002
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2020
derived from same individual: ATCC CRL-7636, Hs 895
