Hs 822.T细胞
是否是肿瘤细胞： 1
物种来源： 人
ATCC Number： CRL-7556™
相关**： 其他**
细胞形态： 上皮样
运输方式： 冻存运输
生长状态： 贴壁生长
年限： 9 years
器官来源： 骨
数量： 大量
Hs 822.T细胞Designations: Hs 822.T
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: bone
Disease: Ewing's sarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 822.T细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 9 years
Gender: female
Ethnicity: Caucasian
Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Hs 822.T细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: Hs 822.T细胞liquid nitrogen vapor phase
