Hs 729细胞
生长状态： 贴壁生长
ATCC Number： HTB-153™
数量： 大量
相关**： 横纹肌肉瘤
年限： 74 years
细胞形态： 成纤维样
组织来源： muscle
运输方式： 冻存运输
是否是肿瘤细胞： 1
物种来源： 人
Hs 729细胞Designations: Hs 729 [Hs 729T]
Depositors: RB Owens
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Tissue: muscle
Disease: rhabdomyosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 729细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,12
D16S539: 11
D5S818: 11,12
D7S820: 8,9
THO1: 6,9.3
TPOX: 11
vWA: 16,17
Isoenzymes: G6PD, B
Age: 74 years
Gender: male
Ethnicity: Caucasian
Hs 729细胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; Hs 729细胞DMSO, 5%
