Hs 696细胞
年限： 43 years
是否是肿瘤细胞： 1
物种来源： 人
ATCC Number： HTB-151™
相关**： 腺癌
细胞形态： 上皮样
生长状态： 贴壁生长
运输方式： 冻存运输
数量： 大量
Designations: Hs 696
Depositors: RB Owens
Hs 696细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Disease: adenocarcinoma
Derived from metastatic site: bone (sacrum)
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Tumorigenic: No
Cytogenetic Analysis: Y chromosome present
Isoenzymes: AK-1, 1
ES-D, 1
Hs 696细胞G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 43 years
Gender: male
Ethnicity: Asian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: Hs 696细胞A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412
22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. Hs 696细胞PubMed: 283258
