NCI-H498细胞
生长状态： 悬浮生长，少量贴壁
运输方式： 冻存运输
数量： 大量
器官来源： 盲肠
年限： 56 years
细胞形态： 上皮样
是否是肿瘤细胞： 1
物种来源： 人
ATCC Number： CCL-254™
相关**： 大肠癌
Designations: NCI-H498 [H498]
NCI-H498细胞Depositors: AF Gazdar
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, with some loosely adherent cells
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: cecum
Disease: colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA), 826 ng/ml per 10 exp6 cells per 10 days; mucin
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Tumorigenic: Yes
Antigen Expression: Rh+; CA19-9 antigen; TAG-72 antigen
DNA Profile (STR): NCI-H498细胞Amelogenin: X
CSF1PO: 12
D13S317: 9,11
D16S539: 9,11
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 15
Cytogenetic Analysis: modal number = 51; range = 47 to 54.
This is a hyperdiploid human cell line. The modal chromosome number is 51, occurring in 42% of the cells. The rate of higher ploidies is 5%. The 2q+ is the only marker chromosome detected and common to all cells. There are four copies for N7; 3 copies each for N8, N13 and N22, and a single copy for the X.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1-2
NCI-H498细胞Age: 56 years
Gender: male
Ethnicity: Caucasian
Comments: This line was derived from a metastasis in the peritoneum obtained from a patient prior to therapy.
The cells form glandular structures and secrete mucin.
The cells are positive for Dopa decarboxylase, CA19-9 and TAG-72 antigens and CEA.
Although the patient from which this cell was derived was blood type A, the cell line does not react with anti type B antibody and has very low to undetectable levels of alpha-N-acetylgalactosaminyltransferase activity.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Alternatively, the cells may be collected by centrifugation and then resuspended at 2 to 4 X 10(4) viable cells/ml. When the cell concentration becomes heavy with large clumps, aspirate well and and dilute approximately 1:10. If the clumps are allowed to get too large, most of the cells will die.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: NCI-H498细胞Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249
23074: Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345
