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NCI-H508细胞

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  • 产品名称:NCI-H508细胞
  • 产品型号:NCI-H508
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-06-18
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简单介绍
NCI-H508细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。NCI-H508细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

NCI-H508细胞

生长状态: 漂浮生长

器官来源: 盲肠

是否是肿瘤细胞: 1

物种来源: 人

数量: 大量

细胞形态: 上皮样

年限: 55 years

ATCC Number: CCL-253™

相关**: 大肠癌

运输方式: 冻存运输

Designations: NCI-H508 [H508]

NCI-H508细胞Depositors: AF Gazdar

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: floating aggregates of round cells with some attached cells

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: cecum

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA), 1709 ng/ml per 10 exp6 cells per 10 days

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.

Tumorigenic: Yes

Antigen Expression: NCI-H508细胞Blood type A; Rh+; CA19-9 antigen

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 8,12

D16S539: 12

D5S818: 12

D7S820: 8,12

THO1: 9,9.3

TPOX: 8,11

vWA: 15,16

Cytogenetic Analysis: modal number = 102; range = 71 to 131; most cells have DM's

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1

Age: 55 years

Gender: male

Ethnicity: NCI-H508细胞Caucasian

Comments: This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.

The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 3 times per week

Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for two minutes. Remove the trypsin and let the flask sit at room temperature for 10 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 23066: Park JG, et al. NCI-H508细胞Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249

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