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NCI-H1385细胞

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  • 产品名称:NCI-H1385细胞
  • 产品型号:NCI-H1385
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-08-11
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简单介绍
NCI-H1385细胞应如何避免细胞污染,细胞污染的种类可分成**、酵母菌、霉菌、病毒和霉浆菌。主要的污染原因为无菌操作技术不当、操作室环境不佳、污染之血清和污染之细胞等。严格之无菌操作技术、清洁的环境、与品质良好之细胞来源和培养基配制是减低污染之*好方法。NCI-H1385细胞何时须更换培养基?视细胞生长密度而定,或遵照细胞株基本数据上之更换时间,按时更换培养基即可。
产品描述

NCI-H1385细胞

年限: stage 3A

器官来源: 肺

数量: 大量

运输方式: 冻存运输

是否是肿瘤细胞: 1

物种来源: 人

ATCC Number: CRL-5867™

相关**: 非小细胞肺癌

生长状态: 悬浮生长

Designations: NCI-H1385 [H1385]

Depositors: AF Gazdar, JD Minna

NCI-H1385细胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology:

Source: Organ: lung

Tumor Stage: stage 3A

Disease: carcinoma; non-small cell lung cancer

Derived from metastatic site: lymph node

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: NCI-H1385细胞The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 11

D16S539: 9,11

D5S818: 13

D7S820: 8,10

THO1: 7

TPOX: 8,9

vWA: 17,19

Age: 49 years

Gender: female

Ethnicity: Black

Comments: NCI-H1385细胞The line was established in April 1986.

The patient was a smoker.

33 pack years.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23570: . NCI-H1385细胞NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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