UMB1949细胞
数量： 大量
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 人
年限： 36 years
细胞形态： 上皮样
组织来源： angiomyolipoma
生长状态： 贴壁生长
ATCC Number： CRL-4004™
相关**： 其他**
器官来源： 肾脏
Designations: UMB1949
Depositors: JL Arbiser
Biosafety Level: 2 UMB1949细胞[cells contain SV40 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial-like
Source: Organ: kidney
Tissue: angiomyolipoma
Disease: tuberous sclerosis
Immortalization method: introduction of telomerase into SV40-transfected angiomyolipoma cells
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Isolation: Isolation date: June 2004
Applications: UMB1949细胞In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings.
As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation.
As such, this cell line can be used to study signal transduction and drug efficiency in tuberous sclerosis.
The UMB1949 cell line was established by sequential introduction of the SV40 large T antigen and human telomerase into human angiomyolipoma cells.
Antigen Expression: positive for epithelial marker pan-cytokeratin (immunocytochemistry)
DNA Profile (STR): Amelogenin: XY
CSF1PO: 10, 11
D13S317: 12, 13
D16S539: 12
D5S818: 11
D7S820: 9, 10
THO1: 6, 8
TPOX: 12
vWA: 18
Cytogenetic Analysis: UMB1949细胞This cell line is of male origin and 1/2 to 2/3 of the total cell population is pseudodiploid, the rest of the cells fall in the tetraploid range. Consistent cytogenetic changes include chromosome 10 and 19 aberration, and chromosome 4 monosomy. Some cells showed loss of the Y chromosome and many of the examined cells contained random chromosomal aberrations.
Age: 36 years
Gender: male
Ethnicity: Caucasian
Comments: The UMB1949 cell line was established by sequential introduction of the SV40 large T antigen and human telomerase into human angiomyolipoma cells. Angiomyolipomas are benign tumors of the kidney which originate from putative perivascular epithelioid cells that may undergo differentiation into cells with features of melanocytes, smooth muscle or fat cells. UMB1949 cells have a defined 5bp deletion in exon 33 of tuberin (Tsc2) and mutations in tuberin (and/or hamartin) cause tuberous sclerosis. As such, this cell line can be used to study signal transduction and drug efficiency in tuberous sclerosis. [16173554]
As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: UMB1949细胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37.0°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1.5 X 104 to 2.5 X 104 viable cells/cm2 is recommended.
Incubate cultures at 37.0°C. Subculture when the cell concentration is between 5 X 104 to 7 X 104 cells/cm2.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:5 is recommended.
Medium renewal: Every 2 to 3 days
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: about 29 hours
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002
Recommended serum: ATCC 30-2020
0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101
Cell culture tested DMSO: ATCC 4-X
Phosphate-buffered saline: ATCC 30-2200
References: 47354: Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332
53507: Arbiser JL, et al. The generation and characterization of a cell line derived from a sporadic renal angiomyolipoma: use of telomerase to obtain stable populations of cells from benign neoplasms. Am. J. Pathol. 159: 483-491, 2001. PubMed: 11485907
16173554: Lim SD , et al. Expression of the neural stem cell markers NG2 and L1 in human angiomyolipoma: are angiomyolipomas neoplasms of stem cells? Mol. Med. 13(3-4): 160-165, 2007. PubMed: 17592550
