The pRSET-EmGFP, pRSET-CFP and pRSET-BFP vectors allow you to fuse a protein of interest to the widely used and well-characterized fluorescent proteins from the jellyfish Aequorea victoria (1,2) using the pRSET expression vector. These vectors contain the next-generation EGFP, Emerald Green Fluorescent Protein (EmGFP), which has been further optimized for mammalian expression, as well as Cyan (CFP) and Blue (BFP) Fluorescent Proteins for simple, noninvasive detection of recombinant protein.