pMXB10 is a control plasmid for the IMPACT Kit (NEB #E6901) (1,2). This plasmid carries the
E. coli malE gene, encoding the maltose binding protein (MBP, 42 kDa) (3), fused in-frame to
the coding region of the Mxe gyrase A intein-chitin binding domain (28 kDa) from pTXB1 (2,4,5).
pMXB10 can be used to test plasmid transformation, cell culture, induction and purification
procedures. After induction with 0.3 mM IPTG at 30°C for 3 hours (or 15°C for 12-16 hours),
100 ml of cells should yield 2-3 mg of a 70 kDa fusion protein. After chitin column purification
and cleavage, approximately 1.5-2.0 mg of MBP is usually obtained. This double stranded vector
is 7813 bp in length.
