pMCS-Green Renilla Luc 载体简介 The Thermo Scientific pMCS-Renilla Luc vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter. pMCS-Renilla Luc 载体特征: Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
High-copy pUC bacterial DNA replication origin
The Green Renilla Luc Vectors contain a gene cloned from the sea pansy, Renilla reniformis. The gene encodes a bioluminescent Green Renilla luciferase (36kDa). The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.
These vectors are subject to a limited use label license.