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pCMV-tdTomato

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  • 产品名称:pCMV-tdTomato
  • 产品型号:
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2018-08-14
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简单介绍
pCMV-tdTomato的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到pCMV-tdTomato后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
产品描述

pCMV-tdTomato载体基本信息

载体名称: pCMV-tdTomato
质粒类型: 哺乳动物细胞表达载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 5392 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
筛选标记: 新霉素(Neomycin)
克隆菌株: DH5α, HB101
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 哺乳动物载体pCMV-tdTomato组成型表达tdTomato蛋白。
产品目录号: 632534
稳定性: 稳表达 或 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pCMV-tdTomato载体质粒图谱和多克隆位点信息

pCMV-tdTomato载体图谱
pCMV-tdTomato 载体特征

pCMV-tdTomato载体简介

pCMV-tdTomato载体描述 pCMV-tdTomato encodes tdTomato, a member of the family of fruit fluorescent proteins (1) derived from the Discosoma sp. red fluorescent protein, DsRed (2). Because the Tomato protein has a tendency to dimerize, the vector was designed with two copies of the Tomato coding region linked together to allow intramolecular dimerization. As a result, each tdTomato RNA transcript encodes a tandem dimer of the Tomato protein (excitation and emission maxima equal 554nm and 581nm, respectively). The tdTomato coding sequence is positioned just downstream of the constitutively active cytomegalovirus immediate early promoter (PCMV IE). As a result, mammalian cells transfected with this vector will constitutively express tdTomato. A Kozak consensus sequence is located immediately upstream of the tdTomato coding sequence to enhance translational efficiency in eukaryotic cells (3). SV40 polyadenylation signals downstream of the tdTomato coding sequence direct proper processing of the 3' end of the tdTomato mRNA. The vector backbone contains an SV40 origin for replication in mammalian cells expressing the SV40 large T antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin resistance cassette (Neor) allows stably transfected eukaryotic cells to be selected using G418. This cassette consists of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene. A bacterial promoter upstream of the cassette expresses kanamycin resistance in E. coli.

pCMV-tdTomato is designed for use as a marker for cotransfection or cell tracking. The red fluorescence of tdTomato can be detected by fluorescence microscopy, allowing direct visual imaging. In addition, flow cytometry can be used to enrich transfected cell populations. pCMV-tdTomato can be transfected into mammalian cells using any standard transfection method. If required, stable transfectants can be selected using G418 (4).

Propagation in E. coli
 Suitable host strains: DH5α, HB101 and other general purpose strains. Single-stranded DNA production requires
a host containing an F plasmid such as JM109 or XL1-Blue.
 Selectable marker: plasmid confers resistance to kanamycin (50 μg/ml) to E. coli hosts.
 E. coli replication origin: pUC
 Copy number: ~500
 Plasmid incompatibility group: pMB1/Col E1

Excitation and emission maxima of tdTomato
 Excitation maximum = 554 nm
 Emission maximum = 581 nm References 1. Shaner, N. C., et al. (2004) Nature Biotech. 22(12):1567-1572.
2. Bevis, B. J. & Glick, B. S. (2002) Nature Biotech. 20:83–87.
3. Kozak, M. (1987) Nucleic Acids Res. 15:8125–8148.
4. Gorman, C. (1985) In DNA cloning: A Practical Approach, Vol. II. Ed. D. M. Glover. (IRL Press, Oxford, U.K.), pp. 143–190. 

pCMV-tdTomato载体序列

hz-6795R CHAC1  阳离子转运调控样蛋白1抗体
hz-6796R CIDEC  细胞死亡活化蛋白抗体
hz-6797R CLPTM1L/CRR9  顺氯氨铂耐药相关蛋白9抗体
hz-6798R Calpain 12  钙激活蛋白酶12抗体
hz-6799R Coronin 3  冠蛋白3抗体
hz-1223R Aggrecan1  软骨蛋白聚糖抗体
hz-5173R phospho-ANAPC1 (Ser355)  磷酸化细胞周期末期促进复合蛋白APC1抗体
hz-6800R DAD1  细胞死亡防卫蛋白1抗体
hz-6801R TCERG1/CA150  转录延伸调节蛋白1抗体
hz-6802R CAB39L  钙结合蛋白样39抗体
hz-3573R Aggrecanase-2/ADAMTS-5  软骨蛋白聚糖抗体
hz-6803R CAGE1/CT3/CT95  肿瘤/睾丸抗原3抗体
hz-6804R CD66d/CEAM3  癌胚抗原相关细胞粘附分子3抗体
hz-6805R CLUAP1  成簇相关蛋白1抗体
hz-6806R CTAG1B/Cancer/testis antigen 1B  肿瘤/睾丸抗原1B
hz-6807R CTAG2  肿瘤/睾丸抗原2抗体
hz-6808R CTAGE5  皮肤T细胞**瘤相关抗原5抗体(脑膜瘤表达抗原6)
hz-5023R AGPAT1  溶血磷脂酰基转移酶抗体
hz-6809R EBAG9/RCAS1  肿瘤相关表面抗原RCAS1抗体
hz-6810R FAM120C  构成激活过氧化酶活化增生受体γ样蛋白2抗体
hz-6811R MUM1/FLJ14868  突变的黑色素瘤相关抗原1抗体
hz-6812R FMNL1  成蛋白相关蛋白1抗体
hz-6813R GAGE7/Cancer/testis antigen family 4 mhzber 7  肿瘤/睾丸抗原家族4亚型7抗体
hz-6814R JAKMIP2/NECC1  JAK和微管相互作用蛋白2抗体(JAK和微管相互作用蛋白2)
hz-6815R LRRC15  富含亮氨酸重复蛋白15抗体
hz-6816R MAGEA10  黑色素瘤相关抗原10抗体

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