pcDNA3.2/V5/GW/D-TOPO载体 The pcDNA3.2/V5/GW/D-TOPO and pcDNA6.2/V5/GW/D-TOPO vectors are designed to facilitate rapid, directional TOPO Cloning of blunt-end PCR products for high-level expression in mammalian cells and for entry into the Gateway Technology. Features of the vectors include:
Human cytomegalovirus immediate-early (CMV) promoter/enhancer for high-level expression in a wide range of mammalian cells
attB1 and attB2 sites for site-specific recombination of the expression clone with a Gateway donor vector to generate an entry clone
Directional TOPO Cloning site for rapid and efficient directional cloning of blunt-end PCR products (see page 3 for more information)
The V5 epitope tag for detection using Anti-V5 antibodies
The Herpes Simplex Virus thymidine kinase polyadenylation signal for proper termination and processing of the recombinant transcript
f1 intergenic region for production of single-strand DNA in F plasmidcontaining E. coli
SV40 early promoter and origin for expression of the neomycin
(pcDNA3.2/V5/GW/D-TOPO) or Blasticidin (pcDNA6.2/V5/GW/DTOPO) resistance gene and stable propagation of the plasmid in mammalian hosts expressing the SV40 large T antigen
Neomycin or Blasticidin resistance gene (pcDNA3.2/V5/GW/D-TOPO and pcDNA6.2/V5/GW/D-TOPO, respectively) for selection of stable cell lines
The pUC origin for high copy replication and maintenance of the plasmid in E. coli
The ampicillin (bla) resistance gene for selection in E. coli
