p3XFLAG-CMV-7 Expression Vector is a 4.7 kb derivative of pCMV5 used to establish expression of N-terminal Met-FLAG fusion proteins in mammalian cells. The vector encodes the ANTI-FLAG M2 antibody epitope (Asp-Tyr-Lys-Xaa-Xaa-Asp) three times, upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2. The third epitope includes the enterokinase recognition sequence allowing cleavage of the 3XFLAG peptide from the purified fusion protein. Efficiency of replication is optimal when using an SV40 T antigen-expressing host.
p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp- Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody.3 The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.
Principle
The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG-fusion constructs. p3XFLAG-CMV-7 Expression Vector is a shuttle vector for E. coli
and mammalian cells.
