载体特征:
hTERT: 4225-7623
HygB: 9216-10238 (complementary)
CatR: 10822-11480
AmpS: 12941-13799 (complementary)
PAC: 368-964 (complementary)
F1 ori: 11501-11685 (complementary)
MPSV: 3643-4119 (promoter)
Ori: 12165-12747
插入基因:
Gene: Telomerase reverse transcriptase (TERT)
Source: Human
Insert size (kb): 3.398
GenBank accession: NM_003219
Medium 2585: LB Medium (ATCC medium 1065) with 37 mcg/ml chloramphenicol
Restriction digests of this clone gave the following sizes (in kb): HindIII/KpnI - 10.5, 3.5; EcoRI - 10.6, 3.4; XhoI - 12.0, 2.0.