The pEF6/V5-His TOPO TA Expression Kit is designed for high-level mammalian
expression from the powerful human EF-1 α promoter with the added convenience
of one-step TOPO Cloning. The pEF6/V5-His-TOPO vector includes:
Enhancer/promoter elements from the human elongation factor 1α
subunit (hEF-1α) for high-level expression in mammalian cells
The Blasticidin resistance gene for rapid selection of stable
mammalian cell lines
A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
A C-terminal polyhistidine (6xHis) sequence for rapid purification
with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
