The pShooter vectors are designed to localize recombinant proteins to the nucleus, mitochondria,
endoplasmic reticulum, or cytoplasm. In addition to the localization signal, each pShooter vector contains:
Strong mammalian promoter (CMV or EF-1a) for constitutive expression of your protein of interest
C-terminal c-myc epitope for detection with an Anti-myc Antibody
Bovine Growth Hormone (BGH) polyadenylation site for mRNA stability
f1 origin for single-stranded rescue of sense DNA
Neomycin resistance gene for selection of mammalian cells with Geneticin selection agent
Ampicillin resistance and pUC origin for selection and maintenance in E. coli
Each vector is provided with a positive control plasmid. The positive control expresses GFP and targets
it to the same subcellular location as the pShooter vector.
