The InsectSelect Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression
• The Zeocin resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistance protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells.
Promoter: OplE2
Vector Type: pIZ
Product Size: 1 kit
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: V5 Epitope Tag, His Tag (6x)
Protein Tag Position (to your gene): C-terminal
