pVL1393载体和pVL1392载体简介 pVL1392 and pVL1393 are 9.8 kb vectors designed for use as baculovirus transfer vectors. These transfer vectors contain recombination sequences which are homologous to sequences in the baculovirus genome.
Upon cotransfection, these sequences undergo homologous recombination resulting in a recombinant viral genome packaged in infectious viral particles.
These vectors are nonfusion vectors, which provide the AcMNPV polyhedrin enhancer-promoter sequences to drive high expression, and a multiple cloning site, conveniently constructed in opposite orientations, for simple subcloning. Translation initiation sequences must be provided by the inserted cDNA. 载体特征 • Multiple cloning site polylinker, positioned in opposite orientations
• Recombination sequences for insertion into the baculovirus genome
• Polyhedrin enhancer-promoter sequences for high expression of recombinant protein
Features for growth and maintenance in E. coli:
• Ampicillin gene for selection
• pMB1 origin of replication from pUC19 for high copy number growth
E. coli Strains
The TOP10 cells are provided as a stab.
TOP10 is provided for growth and maintenance of this plasmid. TOP10 is a recombination-negative strain designed for stable replication of high copy number plasmids. This strain is provided as a convenience for those who do not have access to other E. coli strains. Many E. coli strains are suitable for the growth of this vector including DH5α, DH10, and INVαF'. In general, it is best to grow vectors containing inserts in E. coli strains which are recombination-deficient (recA-).
Genotype of TOP10
F-, mcrA ∆(mrr-hsdRMS-mcrBC) Φ80lacZ∆M15 ∆lacX74 deoR recA1 araD139 ∆(ara-leu)7697 galU galK rpsL endA1 nupG
