pXINSCET-DEST39载体优势： Using the Express Insect Kit or the Express Insect Vector Set to achieve protein expression in insect cells has the following advantages:
Non-lytic, continuous protein expression
Minimal degradation of your protein of interest as compared to viral expression systems
Higher protein expression level achieved than other similar non-viral insect expression systems
Choice of transient or stable protein expression
Adapted for use with the Gateway Technology or traditional restriction enzyme-mediated cloning pXINSCET-DEST39载体特征： The silkmoth (Bombyx mori) cytoplasmic actin gene promoter for constitutive expression of the gene of interest (Johnson et al., 1992)
The viral transcriptional enhancer, homologous region 3 (HR3) from Bombyx mori nuclear polyhedrosis virus (BmNPV) acts as a constitutive enhancer of the cytoplasmic actin promoter (Lu et al., 1997)
A viral transcriptional transactivator, BmNPV IE1, to stimulate expression of your gene of interest (Lu et al., 1996)
N-terminal 6xHis tag fusion proteins to facilitate detection and purification of your protein of interest using pXINSECT-DEST39
Two recombination sites, attR1 and attR2, for recombinational cloning of the gene of interest from an entry clone
Chloramphenicol resistance gene located between the two attR sites for counterselection
The ccdB gene located between the two attR sites for negative selection
The actin polyadenylation signal for efficient transcription termination and polyadenylation of mRNA
Ampicillin resistance gene for selection of transformants in E. coli
The pUC origin for high copy replication and maintenance of the plasmid in E. coli
