The DES TOPO TA Expression Kit offers one-step cloning of PCR products directly into an inducible DES expression vector. The kit uses the linearized, topoisomerase I-activated pMT/V5-His-TOPO vector for fast and easy cloning and subsequent high-level expression. Topoisomerase activation of this vector allows PCR products to be ligated in just 5 minutes on your bench top to produce >85%recombinants. The pMT/V5-His-TOPO vector has all of the features of the pMT/V5-His vector to simplify protein expression, detection, and purification of recombinant proteins in Drosophila S2 cells.
引用及参考文献
Analysis of Drosophila cGMP-dependent protein kinases and assessment of their in vivo roles by targeted expression in a renal transporting epithelium.
MacPherson MR, Lohmann SM, Davies SA, J Biol Chem (2004) 279:40026-40034
Product usage: Primers specific for the DG1 gene transcript (cGMP-dependent protein kinase (cGK) forms) and each of the two major DG2 gene transcripts were
used to create PCR products encompassing the appropriate open reading frames. These were sequenced and cloned into the pMT/V5-HIS-TOPO vec
