pCoBlast is a 3907 bp selection vector that can be cotransfected with the expression vector of choice to to create stable cell lines in Drosophila. It contains the Streptomyces griseochromogenes bsd gene under control of the Drosophila copia promoter to confer resistance to blasticidin in S2 cells.
引用及参考文献
A novel allelic variant of the human TSG-6 gene encoding an amino acid difference in the CUB module. Chromosomal localization, frequency analysis, modeling, and expression.
Nentwich Hilke A; Mustafa Zehra; Rugg Marilyn S; Marsden Brian D; Cordell Martin R; Mahoney David J; Jenkins Suzanne C; Dowling Barbara; Fries Erik; Milner Caroline M; Loughlin John; Day Anthony J;
J Biol Chem (2002) 277:15354-15416
Product usage: The full-length coding sequence of human TSG-6 (A431 allele encoding Gln at amino acid 144), including the signal sequence and stop codon, was excised from the pAcCL29-1 vector by digestion with KpnI/XbaI and cloned into the corresponding sites in the Drosophila Expression System
