The pIEx/Bac-3 vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in
Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV)
enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV
p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions
to mediate recombination at the polh locus. The pIEx/Bac-3 vector contains an N-terminal His•Tag® coding sequence with 10
histidines followed by an HRV 3C protease cleavage site and a multiple cloning site, with the option to include a C-terminal
thrombin protease cleavage site and 8 aa Strep•Tag® II coding sequence. pIEx/Bac-3 is also available as a 3C/LIC vector. The
3C/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for
amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.
