pCTAP-Shuttle-C载体限制性酶切位点
pCTAP Shuttle C, 7710 bp version 016001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AarI 1 3153
AatII 4 459, 512, 595, 781
Acc65I 1 940
AccI 3 946, 2790, 3728
AccIII 3 1136, 3883, 4110
AclI 1 6089
AcuI 6 1766, 1963, 3369, 5498, 6705
7137
AflIII 4 143, 980, 3923, 4971
AhdI 2 2089, 5859
Alw44I 2 2473, 5285
AlwNI 5 1769, 1927, 2012, 5382, 6199
ApaI 2 2583, 2889
ApoI 7 242, 1159, 1408, 2484, 3756
7245, 7256
AseI 3 4253, 6035, 7695
AvaI 4 959, 1904, 2084, 4188
AvaII 7 2088, 2112, 3137, 3278, 3356
6002, 7096
BamHI 1 4788
BanII 10 1109, 1132, 1605, 1662, 1818
2583, 2889, 3608, 6941, 7514
BbeI 3 3482, 6331, 6579
BbsI 2 1009, 3383
BciVI 3 2466, 5180, 6788
BclI 2 1251, 6421
BfaI 10 930, 1242, 1443, 4299, 5466
5719, 6054, 6139, 6281, 7594
BfrBI 3 2379, 2557, 4782
BglI 7 422, 544, 615, 1047, 1960
4038, 5978
BglII 1 934
BlnI 1 4298
Bme1580I 7 1212, 2473, 2583, 2889, 5285
6522, 6615
BmgBI 3 65, 2133, 4703
BmrI 5 632, 5909, 6204, 6285, 6519
BpmI 5 1061, 1082, 1665, 4322, 5949
Bpu10I 4 965, 1815, 2859, 2955
BpuEI 6 3347, 3737, 5077, 5339, 5616
6971
BsaAI 6 676, 1034, 2363, 6151, 6882
7441
BsaBI 4 1252, 2025, 4150, 6435
BsaHI 8 459, 512, 595, 781, 2529
3482, 6331, 6579
BsaI 5 1720, 2096, 3084, 3584, 5931
BsaWI 9 1136, 1235, 2469, 3883, 4110
5177, 5324, 6313, 6611
BseMII 9 1095, 1174, 1816, 1925, 2860
2956, 5246, 5655, 5821
BseRI 3 3185, 3477, 4097
BseYI 9 1129, 2261, 2348, 2415, 2840
3037, 5275, 6326, 7084
BsgI 4 1835, 2445, 3500, 4456
BsiEI 7 953, 987, 2101, 4176, 4884
5308, 6486
BsiHKAI 9 1085, 1575, 1605, 2473, 3233
3608, 5285, 6689, 6879
BsmBI 3 1171, 2180, 2527
BsmFI 8 499, 650, 818, 2087, 2985
3138, 3722, 6717
BsmI 4 1339, 1438, 2559, 3590
BspCNI 9 1095, 1174, 1816, 1925, 2860
2956, 5246, 5655, 5821
BspHI 1 5691
BspMI 8 1950, 2794, 2852, 3154, 3873
6247, 6476, 6839
BsrBI 5 956, 4319, 4902, 7191, 7585
BsrDI 5 998, 2578, 5918, 6100, 6806
BsrGI 1 192
BssHII 5 2070, 2072, 3328, 3459, 7644
BssSI 4 3489, 3596, 5144, 7172
Bst1107I 1 3728
BstAPI 2 1828, 3047
BstEII 2 3150, 4066
BstXI 1 2567
Bsu36I 1 1094
BtgI 8 698, 1633, 1788, 2587, 3266
3418, 3442, 7012
BtgZI 7 687, 1859, 2803, 6866, 7017
7210, 7429
BtsI 5 1434, 1832, 2203, 3497, 6258
ClaI 1 974
Csp6I 10 193, 556, 581, 636, 669
720, 877, 941, 3218, 6885
DraI 6 1294, 1991, 3749, 3973, 5728
5747
DraIII 2 1034, 7438
DrdI 4 3416, 5073, 6601, 7392
EagI 2 953, 6486
EarI 3 4849, 6929, 7139
Eco47III 3 2258, 3373, 4338
EcoICRI 2 1605, 3608
EcoO109I 5 1044, 2111, 2314, 3277, 3571
EcoRI 1 3756
EcoRV 1 1223
FspI 2 6084, 6680
HgaI 9 855, 949, 982, 1647, 2529
3396, 5073, 5651, 7670
HincII 3 946, 1355, 1869
HindIII 1 968
HpaI 1 1355
KasI 3 3482, 6331, 6579
KpnI 1 940
MluI 1 980
MmeI 5 1740, 1935, 5161, 5345, 7395
MscI 3 268, 3334, 6660
MslI 5 699, 2568, 2633, 3854, 7013
MunI 2 1344, 1888
NaeI 4 1052, 4043, 7080, 7544
NarI 3 3482, 6331, 6579
NcoI 4 698, 1633, 3418, 7012
NdeI 2 571, 4793
NgoMIV 4 1052, 4043, 7080, 7544
NotI 1 952
NsiI 3 2379, 2557, 4782
NspI 9 143, 1625, 2981, 2996, 3101
3923, 4780, 4971, 6981
PacI 2 4773, 7703
PciI 3 143, 3923, 4971
PfoI 2 2128, 2644
PmeI 1 3748
PmlI 1 1034
PpuMI 2 2111, 3277
PsiI 3 1375, 4015, 7313
PspOMI 2 2583, 2889
PstI 5 1748, 1775, 2205, 2938, 6105
PvuI 1 987
PvuII 7 1067, 1930, 2838, 2865, 2941
6324, 6684
RsaI 10 193, 556, 581, 636, 669
720, 877, 941, 3218, 6885
RsrII 1 7095
SacI 2 1605, 3608
SacII 2 1788, 3442
SalI 1 946
SapI 3 4848, 6929, 7139
SexAI 1 3633
SfcI 8 1748, 1775, 2205, 2938, 5236
5427, 6105, 7661
SfoI 3 3482, 6331, 6579
SgrAI 1 186
SmaI 3 1904, 2084, 4188
SmlI 7 959, 3347, 3737, 5077, 5339
5616, 6971
SnaBI 1 676
SphI 3 1625, 3101, 6981
StyI 9 698, 1633, 2318, 3418, 3454
3472, 4298, 6389, 7012
TatI 5 192, 555, 635, 668, 719
TfiI 7 1894, 2435, 3543, 4150, 4946
7065, 7199
TspGWI 4 154, 3717, 4028, 4590
Tth111I 2 1722, 6695
Van91I 3 1014, 1033, 2129
XbaI 1 1241
XcmI 3 2011, 3602, 6389
XhoI 1 959
XmaI 3 1904, 2084, 4188
XmnI 1 7693
ZraI 4 459, 512, 595, 781
Enzymes that do NOT cut molecule:
AflII AgeI AleI AscI AsiSI
BbvCI BlpI BmtI BsiWI BstBI
EcoNI FseI FspAI NheI NruI
PshAI SanDI SbfI ScaI SfiI
SpeI SrfI SspI StuI SwaI
