pNTAP-Shuttle-A载体限制性酶切位点
pNTAP Shuttle A, 7735 bp version 016001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AarI 1 3178
AatII 4 459, 512, 595, 781
Acc65I 1 1039
AccI 3 1201, 2815, 3753
AccIII 2 3908, 4135
AclI 1 6114
AcuI 6 1791, 1988, 3394, 5523, 6730
7162
AflIII 4 143, 1235, 3948, 4996
AhdI 2 2114, 5884
Alw44I 2 2498, 5310
AlwNI 5 1794, 1952, 2037, 5407, 6224
ApaI 2 2608, 2914
ApoI 7 242, 966, 1433, 2509, 3781
7270, 7281
AseI 3 4278, 6060, 7720
AvaI 6 1181, 1194, 1214, 1929, 2109
4213
AvaII 7 2113, 2137, 3162, 3303, 3381
6027, 7121
BamHI 2 1188, 4813
BanII 9 1153, 1630, 1687, 1843, 2608
2914, 3633, 6966, 7539
BbeI 4 1177, 3507, 6356, 6604
BbsI 2 1053, 3408
BciVI 3 2491, 5205, 6813
BclI 2 1276, 6446
BfrBI 3 2404, 2582, 4807
BglI 7 422, 544, 615, 1091, 1985
4063, 6003
BglII 1 934
BlnI 1 4323
Bme1580I 7 1019, 2498, 2608, 2914, 5310
6547, 6640
BmgBI 3 65, 2158, 4728
BmrI 5 632, 5934, 6229, 6310, 6544
BmtI 1 940
BpmI 5 1105, 1126, 1690, 4347, 5974
Bpu10I 3 1840, 2884, 2980
BpuEI 6 3372, 3762, 5102, 5364, 5641
6996
BsaAI 7 676, 1078, 2388, 4630, 6176
6907, 7466
BsaBI 4 1277, 2050, 4175, 6460
BsaHI 10 459, 512, 595, 781, 1177
1198, 2554, 3507, 6356, 6604
BsaI 5 1745, 2121, 3109, 3609, 5956
BsaWI 8 1260, 2494, 3908, 4135, 5202
5349, 6338, 6636
BseMII 9 981, 1139, 1841, 1950, 2885
2981, 5271, 5680, 5846
BseRI 3 3210, 3502, 4122
BseYI 9 1173, 2286, 2373, 2440, 2865
3062, 5300, 6351, 7109
BsgI 4 1860, 2470, 3525, 4481
BsiEI 7 1208, 1242, 2126, 4201, 4909
5333, 6511
BsiHKAI 9 1129, 1600, 1630, 2498, 3258
3633, 5310, 6714, 6904
BsmBI 3 978, 2205, 2552
BsmFI 8 499, 650, 818, 2112, 3010
3163, 3747, 6742
BsmI 4 1364, 1463, 2584, 3615
BspCNI 9 981, 1139, 1841, 1950, 2885
2981, 5271, 5680, 5846
BspHI 1 5716
BspMI 8 1975, 2819, 2877, 3179, 3898
6272, 6501, 6864
BsrBI 5 1211, 4344, 4927, 7216, 7610
BsrDI 4 2603, 5943, 6125, 6831
BsrGI 1 192
BssHII 5 2095, 2097, 3353, 3484, 7669
BssSI 4 3514, 3621, 5169, 7197
Bst1107I 1 3753
BstAPI 2 1853, 3072
BstEII 2 3175, 4091
BstXI 1 2592
Bsu36I 1 1138
BtgI 9 698, 1043, 1658, 1813, 2612
3291, 3443, 3467, 7037
BtgZI 7 687, 1884, 2828, 6891, 7042
7235, 7454
BtsI 5 1459, 1857, 2228, 3522, 6283
ClaI 1 1229
Csp6I 10 193, 556, 581, 636, 669
720, 877, 1040, 3243, 6910
DdeI 10 981, 1139, 1841, 1950, 2524
2885, 2981, 5271, 5680, 5846
DraI 6 1319, 2016, 3774, 3998, 5753
5772
DraIII 2 1078, 7463
DrdI 4 3441, 5098, 6626, 7417
EagI 2 1208, 6511
EarI 3 4874, 6954, 7164
Eco47III 3 2283, 3398, 4363
EcoICRI 2 1630, 3633
EcoO109I 5 1088, 2136, 2339, 3302, 3596
EcoRI 1 3781
EcoRV 1 1248
FspI 2 6109, 6705
HgaI 10 855, 1199, 1204, 1237, 1672
2554, 3421, 5098, 5676, 7695
HincII 3 1201, 1380, 1894
HindIII 1 1223
HpaI 1 1380
KasI 4 1177, 3507, 6356, 6604
KpnI 1 1039
MluI 1 1235
MmeI 5 1765, 1960, 5186, 5370, 7420
MscI 3 268, 3359, 6685
MslI 5 699, 2593, 2658, 3879, 7038
MunI 2 1369, 1913
NaeI 4 1096, 4068, 7105, 7569
NarI 4 1177, 3507, 6356, 6604
NcoI 5 698, 1043, 1658, 3443, 7037
NdeI 2 571, 4818
NgoMIV 4 1096, 4068, 7105, 7569
NheI 1 940
NotI 1 1207
NsiI 3 2404, 2582, 4807
NspI 9 143, 1650, 3006, 3021, 3126
3948, 4805, 4996, 7006
PacI 2 4798, 7728
PciI 3 143, 3948, 4996
PfoI 2 2153, 2669
PmeI 1 3773
PmlI 1 1078
PpuMI 2 2136, 3302
PsiI 3 1400, 4040, 7338
PspOMI 2 2608, 2914
PstI 5 1773, 1800, 2230, 2963, 6130
PvuI 1 1242
PvuII 7 1111, 1955, 2863, 2890, 2966
6349, 6709
RsaI 10 193, 556, 581, 636, 669
720, 877, 1040, 3243, 6910
RsrII 1 7120
SacI 2 1630, 3633
SacII 2 1813, 3467
SalI 1 1201
SapI 3 4873, 6954, 7164
SexAI 1 3658
SfcI 8 1773, 1800, 2230, 2963, 5261
5452, 6130, 7686
SfoI 4 1177, 3507, 6356, 6604
SgrAI 1 186
SmaI 5 1181, 1194, 1929, 2109, 4213
SmlI 7 1214, 3372, 3762, 5102, 5364
5641, 6996
SnaBI 2 676, 4630
SphI 3 1650, 3126, 7006
SrfI 1 1180
StyI 10 698, 1043, 1658, 2343, 3443
3479, 3497, 4323, 6414, 7037
TatI 5 192, 555, 635, 668, 719
TfiI 7 1919, 2460, 3568, 4175, 4971
7090, 7224
TspGWI 4 154, 3742, 4053, 4615
Tth111I 2 1747, 6720
Van91I 3 1058, 1077, 2154
XbaI 1 1266
XcmI 3 2036, 3627, 6414
XhoI 1 1214
XmaI 5 1181, 1194, 1929, 2109, 4213
XmnI 1 7718
ZraI 4 459, 512, 595, 781
Enzymes that do NOT cut molecule:
AflII AgeI AleI AscI AsiSI
BbvCI BlpI BsiWI BstBI EcoNI
FseI FspAI NruI PshAI SanDI
SbfI ScaI SfiI SpeI SspI
StuI SwaI
