pNTAP-Shuttle-B限制性酶切位点
pNTAP Shuttle B, 7736 bp version 016001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AarI 1 3179
AatII 4 459, 512, 595, 781
Acc65I 1 1039
AccI 3 1202, 2816, 3754
AccIII 2 3909, 4136
AclI 1 6115
AcuI 6 1792, 1989, 3395, 5524, 6731
7163
AflIII 4 143, 1236, 3949, 4997
AhdI 2 2115, 5885
Alw44I 2 2499, 5311
AlwNI 5 1795, 1953, 2038, 5408, 6225
ApaI 2 2609, 2915
ApoI 7 242, 966, 1434, 2510, 3782
7271, 7282
AseI 3 4279, 6061, 7721
AvaI 6 1182, 1195, 1215, 1930, 2110
4214
AvaII 7 2114, 2138, 3163, 3304, 3382
6028, 7122
BamHI 2 1189, 4814
BanII 9 1153, 1631, 1688, 1844, 2609
2915, 3634, 6967, 7540
BbeI 3 3508, 6357, 6605
BbsI 2 1053, 3409
BciVI 3 2492, 5206, 6814
BclI 2 1277, 6447
BfrBI 3 2405, 2583, 4808
BglI 7 422, 544, 615, 1091, 1986
4064, 6004
BglII 1 934
BlnI 1 4324
Bme1580I 7 1019, 2499, 2609, 2915, 5311
6548, 6641
BmgBI 3 65, 2159, 4729
BmrI 5 632, 5935, 6230, 6311, 6545
BmtI 1 940
BpmI 5 1105, 1126, 1691, 4348, 5975
Bpu10I 3 1841, 2885, 2981
BpuEI 6 3373, 3763, 5103, 5365, 5642
6997
BsaAI 6 676, 1078, 2389, 6177, 6908
7467
BsaBI 4 1278, 2051, 4176, 6461
BsaHI 9 459, 512, 595, 781, 1199
2555, 3508, 6357, 6605
BsaI 5 1746, 2122, 3110, 3610, 5957
BsaWI 8 1261, 2495, 3909, 4136, 5203
5350, 6339, 6637
BseMII 9 981, 1139, 1842, 1951, 2886
2982, 5272, 5681, 5847
BseRI 3 3211, 3503, 4123
BseYI 9 1173, 2287, 2374, 2441, 2866
3063, 5301, 6352, 7110
BsgI 4 1861, 2471, 3526, 4482
BsiEI 7 1209, 1243, 2127, 4202, 4910
5334, 6512
BsiHKAI 9 1129, 1601, 1631, 2499, 3259
3634, 5311, 6715, 6905
BsmBI 3 978, 2206, 2553
BsmFI 8 499, 650, 818, 2113, 3011
3164, 3748, 6743
BsmI 4 1365, 1464, 2585, 3616
BspCNI 9 981, 1139, 1842, 1951, 2886
2982, 5272, 5681, 5847
BspHI 1 5717
BspMI 8 1976, 2820, 2878, 3180, 3899
6273, 6502, 6865
BsrBI 5 1212, 4345, 4928, 7217, 7611
BsrDI 4 2604, 5944, 6126, 6832
BsrGI 1 192
BssHII 5 2096, 2098, 3354, 3485, 7670
BssSI 4 3515, 3622, 5170, 7198
Bst1107I 1 3754
BstAPI 2 1854, 3073
BstEII 2 3176, 4092
BstXI 1 2593
Bsu36I 1 1138
BtgI 9 698, 1043, 1659, 1814, 2613
3292, 3444, 3468, 7038
BtgZI 7 687, 1885, 2829, 6892, 7043
7236, 7455
BtsI 5 1460, 1858, 2229, 3523, 6284
ClaI 1 1230
Csp6I 10 193, 556, 581, 636, 669
720, 877, 1040, 3244, 6911
DdeI 10 981, 1139, 1842, 1951, 2525
2886, 2982, 5272, 5681, 5847
DraI 6 1320, 2017, 3775, 3999, 5754
5773
DraIII 2 1078, 7464
DrdI 4 3442, 5099, 6627, 7418
EagI 2 1209, 6512
EarI 3 4875, 6955, 7165
Eco47III 3 2284, 3399, 4364
EcoICRI 2 1631, 3634
EcoO109I 5 1088, 2137, 2340, 3303, 3597
EcoRI 1 3782
EcoRV 1 1249
FspI 2 6110, 6706
HgaI 10 855, 1200, 1205, 1238, 1673
2555, 3422, 5099, 5677, 7696
HincII 3 1202, 1381, 1895
HindIII 1 1224
HpaI 1 1381
KasI 3 3508, 6357, 6605
KpnI 1 1039
MluI 1 1236
MmeI 5 1766, 1961, 5187, 5371, 7421
MscI 3 268, 3360, 6686
MslI 5 699, 2594, 2659, 3880, 7039
MunI 2 1370, 1914
NaeI 4 1096, 4069, 7106, 7570
NarI 3 3508, 6357, 6605
NcoI 5 698, 1043, 1659, 3444, 7038
NdeI 2 571, 4819
NgoMIV 4 1096, 4069, 7106, 7570
NheI 1 940
NotI 1 1208
NsiI 3 2405, 2583, 4808
NspI 9 143, 1651, 3007, 3022, 3127
3949, 4806, 4997, 7007
PacI 2 4799, 7729
PciI 3 143, 3949, 4997
PfoI 2 2154, 2670
PmeI 1 3774
PmlI 1 1078
PpuMI 2 2137, 3303
PsiI 3 1401, 4041, 7339
PspOMI 2 2609, 2915
PstI 5 1774, 1801, 2231, 2964, 6131
PvuI 1 1243
PvuII 7 1111, 1956, 2864, 2891, 2967
6350, 6710
RsaI 10 193, 556, 581, 636, 669
720, 877, 1040, 3244, 6911
RsrII 1 7121
SacI 2 1631, 3634
SacII 2 1814, 3468
SalI 1 1202
SapI 3 4874, 6955, 7165
SexAI 1 3659
SfcI 8 1774, 1801, 2231, 2964, 5262
5453, 6131, 7687
SfoI 3 3508, 6357, 6605
SgrAI 1 186
SmaI 5 1182, 1195, 1930, 2110, 4214
SmlI 7 1215, 3373, 3763, 5103, 5365
5642, 6997
SnaBI 1 676
SphI 3 1651, 3127, 7007
SrfI 1 1181
StyI 10 698, 1043, 1659, 2344, 3444
3480, 3498, 4324, 6415, 7038
TatI 5 192, 555, 635, 668, 719
TfiI 7 1920, 2461, 3569, 4176, 4972
7091, 7225
TspGWI 4 154, 3743, 4054, 4616
Tth111I 2 1748, 6721
Van91I 3 1058, 1077, 2155
XbaI 1 1267
XcmI 3 2037, 3628, 6415
XhoI 1 1215
XmaI 5 1182, 1195, 1930, 2110, 4214
XmnI 1 7719
ZraI 4 459, 512, 595, 781
Enzymes that do NOT cut molecule:
AflII AgeI AleI AscI AsiSI
BbvCI BlpI BsiWI BstBI EcoNI
FseI FspAI NruI PshAI SanDI
SbfI ScaI SfiI SpeI SspI
StuI SwaI
