注:
When cloning your gene of interest into the pLVX-Hom-Mem1 vector, make certain that the N-myr-signal is located on the N-terminus of the DmrB fusion protein. The N‐myr‐signal is NOT functional if localized in the middle or at the C‐terminus of a protein of interest. Your gene must either be cloned into the 3’MCS, or if cloned into the 5’ MCS, which is located upstream of the N‐myr signal, you must include a separate N‐myr signal sequence on the forward primer used to amplify your gene of interest.