The Vivid Colors pLenti6.3-V5-GW-EmGFP Expression Control Vector is a
ViraPower HiPerform positive control lentiviral vector containing
Emerald Green Fluorescent Protein (EmGFP). It is designed for use
with the ViraPower HiPerform Lentiviral Expression Systems as a
positive control to enable the detection of higher levels of
EmGFP fluorescence following transfection in 293FT cells, as a
titer control to produce an EmGFP-expressing lentivirus stock
and as a transduction control following transduction in both
dividing and non-dividing mammalian cells. The vector has the
CMV promoter for driving constitutive expression of EmGFP
and the SV40 promoter for driving expression of the blasticidin
stable selection marker. The vector is equipped with two key
genetic elements, making them Hiperform vectors: the Woodchuck
Posttranscriptional Regulatory Element (WPRE) and the central
Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene
to produce at least 4-fold increase in EmGFP expression compared
to vectors lacking these elements. This control vector is not
designed for generating EmGFP fusion proteins and does not express
the V5 epitope. Advantages Lentivirus based expression of EmGFP in dividing and non-dividing
mammalian cells
Serve as a quick positive control for transfection and lentiviral
production
Serve as a quick titer control in determination of lentivirus titer Key Features Constitutive expression with CMV promoter
High level expression of EmGFP without V5 epitope
WPRE and cPPT sequences produce at least 4-fold increase in
EmGFP expression compared to other
Lenti vectors without these elements
Blasticidin selection marker for stable selection
Kit Includes
pLenti6.3V5-GWEmGFP Expression Control Vector
