pFB-hrGFP载体限制性酶切位点 pFB-hrGFP, 5838 bp version 013001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AatII 2 978, 5764
Acc65I 3 647, 1846, 3364
AccI 3 1967, 2057, 3724
AflII 3 202, 1265, 2919
AflIII 3 164, 2157, 3953
AgeI 1 1840
AhdI 6 687, 733, 1274, 3404, 3450
4841
Alw44I 4 1054, 3769, 4267, 5513
AlwNI 6 323, 398, 2084, 3040, 3115
4364
ApaI 1 1238
ApoI 3 87, 1128, 2063
BanII 8 580, 593, 764, 1238, 2266
3297, 3310, 3481
BbeI 4 615, 1656, 2234, 3332
BbsI 3 2494, 2686, 5831
BbvCI 2 453, 3170
BciVI 5 656, 1986, 3373, 4162, 5689
BglI 1 4960
BglII 1 1678
BpmI 5 1799, 2135, 2681, 2867, 4931
Bpu10I 8 337, 412, 453, 1548, 2263
3054, 3129, 3170
BsaAI 5 1816, 2000, 2156, 2693, 3705
BsaHI 7 615, 978, 1656, 2234, 3332
5382, 5764
BsaI 10 694, 715, 782, 1414, 1802
2726, 3411, 3432, 3499, 4913
BsaWI 5 138, 1840, 4159, 4306, 5137
BseRI 6 718, 1068, 1561, 1600, 2426
3435
BsgI 2 2213, 2774
BsiEI 8 961, 1034, 1838, 2801, 3866
4290, 5213, 5362
BsiHKAI 7 580, 1054, 3297, 3769, 4267
5428, 5513
BsmBI 6 976, 1093, 1337, 1396, 1582
3602
Bsp120I 1 1238
BspHI 4 2115, 4673, 5681, 5786
BspLU11I 2 164, 3953
BsrBI 5 1299, 2798, 2823, 3884, 5685
BsrDI 2 4900, 5082
BsrGI 2 1541, 2532
BssHII 2 563, 3280
BssSI 5 2723, 2777, 4126, 5510, 5817
Bst1107I 1 3724
BstAPI 5 322, 397, 3039, 3114, 3771
BstEII 1 1346
Bsu36I 1 1276
Cfr10I 5 1840, 2099, 2348, 2504, 4926
DraI 3 4710, 4729, 5421
DraIII 1 1873
DrdI 2 3642, 4055
DsaI 4 151, 931, 2070, 2166
EaeI 7 827, 961, 1368, 1389, 1668
2801, 5234
EarI 5 1334, 1564, 2626, 3831, 5635
Ecl136II 2 580, 3297
Eco52I 2 961, 2801
Eco57I 3 2090, 4480, 5528
Eco72I 2 2000, 2156
EcoNI 2 1647, 2419
EcoO109I 5 499, 1477, 1924, 3216, 5821
EcoRI 1 2063
EcoRV 4 308, 383, 3025, 3100
EheI 4 615, 1656, 2234, 3332
FspI 2 2613, 5066
HaeII 6 615, 1656, 2234, 3332, 3827
4197
HgaI 6 889, 1166, 3659, 4055, 4633
5383
HincII 2 2057, 5385
HinfI 10 637, 1020, 1045, 1863, 2829
3354, 3853, 3928, 4324, 4841
KasI 4 615, 1656, 2234, 3332
KpnI 3 647, 1846, 3364
MscI 3 827, 1368, 1668
MslI 6 159, 2068, 2617, 5094, 5253
5612
MunI 2 11, 21
NaeI 1 2348
NarI 4 615, 1656, 2234, 3332
NcoI 2 2070, 2166
NdeI 3 1664, 1672, 3775
NgoMIV 1 2348
NheI 5 6, 16, 26, 197, 2914
NotI 1 2800
NspI 4 164, 2610, 3588, 3953
PleI 9 637, 1020, 1045, 1863, 2829
3354, 3853, 4324, 4841
PpuMI 4 499, 1477, 1924, 3216
PshAI 1 1015
Psp1406I 2 5071, 5444
PstI 5 1178, 1360, 2105, 2510, 5087
PvuI 2 1034, 5213
PvuII 7 286, 361, 2207, 2602, 2741
3003, 3078
SacI 2 580, 3297
SacII 1 151
SalI 1 2057
SapI 2 2626, 3830
SbfI 1 2104
ScaI 1 5324
SchI 9 637, 1020, 1045, 1863, 2829
3354, 3853, 4324, 4841
SexAI 1 1474
SfcI 10 182, 1178, 1360, 2105, 2510
2599, 2899, 4218, 4409, 5087
SmaI 4 643, 1241, 2018, 3360
SmlI 8 202, 1265, 2795, 2919, 4059
4321, 4598, 5466
SpeI 1 897
SrfI 1 1240
SspI 1 5648
StyI 8 496, 706, 1504, 2070, 2166
2229, 3213, 3423
TatI 5 1541, 2451, 2532, 3759, 5324
TfiI 1 3928
Tsp45I 7 1282, 1491, 2225, 3608, 3703
5103, 5314
TspRI 10 1284, 1875, 3849, 4355, 4368
4639, 4788, 4893, 5240, 5267
Tth111I 4 633, 1473, 3350, 3697
VspI 1 5017
XbaI 3 464, 2036, 3181
XcmI 1 2517
XhoI 1 2795
XhoII 10 1678, 2085, 2217, 2400, 4594
4605, 4691, 4703, 5471, 5488
XmaI 4 643, 1241, 2018, 3360
XmnI 2 2644, 5441
Enzymes that do NOT cut molecule:
AccIII AscI BamHI BclI BlnI
Bpu1102I BsaBI BsiWI BsmI BspMI
BstBI BstXI ClaI Eco47III FseI
HindIII HpaI MluI NruI NsiI
PacI PmeI Ppu10I RsrII SanDI
SfiI SgfI SgrAI SnaBI SphI
StuI SwaI Van91I
