pFB-Luc载体限制性酶切位点 pFB-Luc, 6768 bp version 013001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AatII 2 978, 6694
Acc65I 3 647, 1846, 4294
AccI 3 1967, 2057, 4654
AccIII 2 2759, 3275
AflII 3 202, 1265, 3849
AflIII 3 164, 2557, 4883
AgeI 1 1840
AhdI 6 687, 733, 1274, 4334, 4380
5771
Alw44I 4 1054, 4699, 5197, 6443
AlwNI 5 323, 398, 3970, 4045, 5294
ApaI 1 1238
ApoI 5 87, 1128, 2467, 2651, 2905
AvaI 10 577, 610, 643, 1241, 2018
3120, 3725, 4224, 4257, 4290
BamHI 1 3720
BanII 8 580, 593, 764, 1238, 3084
4227, 4240, 4411
BbeI 4 615, 1656, 2096, 4262
BbsI 4 2067, 3345, 3475, 6761
BbvCI 2 453, 4100
BciVI 8 656, 1986, 2762, 3067, 3191
4303, 5092, 6619
BglI 1 5890
BglII 1 1678
BpmI 5 1799, 2128, 3322, 3797, 5861
Bpu10I 7 337, 412, 453, 1548, 3984
4059, 4100
BsaAI 4 1816, 2000, 2216, 4635
BsaHI 9 615, 978, 1656, 2070, 2096
3489, 4262, 6312, 6694
BsaI 9 694, 715, 782, 1414, 1802
4341, 4362, 4429, 5843
BsaWI 9 138, 1840, 2759, 3258, 3275
3642, 5089, 5236, 6067
BseRI 6 718, 1068, 1561, 1600, 3604
4365
BsiEI 9 961, 1034, 1838, 3145, 3731
4796, 5220, 6143, 6292
BsiHKAI 8 580, 1054, 3525, 4227, 4699
5197, 6358, 6443
BsiWI 1 2218
BsmBI 6 976, 1093, 1337, 1396, 1582
4532
Bsp120I 1 1238
BspHI 3 5603, 6611, 6716
BspLU11I 2 164, 4883
BspMI 1 3471
BsrBI 5 1299, 3728, 3753, 4814, 6615
BsrDI 2 5830, 6012
BsrGI 2 1541, 2554
BssHII 2 563, 4210
BssSI 3 5056, 6440, 6747
Bst1107I 1 4654
BstAPI 5 322, 397, 3969, 4044, 4701
BstBI 2 2232, 3020
BstEII 2 1346, 2671
Bsu36I 2 1276, 2676
Cfr10I 4 1840, 2333, 3492, 5856
ClaI 1 3428
DraI 3 5640, 5659, 6351
DraIII 1 1873
DrdI 2 4572, 4985
DsaI 3 151, 931, 2062
EaeI 7 827, 961, 1368, 1389, 1668
3731, 6164
EarI 7 1334, 1564, 2153, 2317, 2871
4761, 6565
Ecl136II 2 580, 4227
Eco52I 2 961, 3731
Eco57I 3 2892, 5410, 6458
Eco72I 1 2000
EcoNI 2 1647, 3677
EcoO109I 6 499, 1477, 1924, 3242, 4146
6751
EcoRI 1 2651
EcoRV 5 308, 383, 3400, 3955, 4030
EheI 4 615, 1656, 2096, 4262
FspI 1 5996
HaeII 6 615, 1656, 2096, 4262, 4757
5127
HincII 3 2057, 3366, 6315
KasI 4 615, 1656, 2096, 4262
KpnI 3 647, 1846, 4294
MscI 3 827, 1368, 1668
MslI 4 159, 6024, 6183, 6542
MunI 4 11, 21, 2179, 2635
NarI 4 615, 1656, 2096, 4262
NcoI 1 2062
NdeI 3 1664, 1672, 4705
NheI 5 6, 16, 26, 197, 3844
NotI 1 3730
NspI 4 164, 2723, 4518, 4883
PacI 1 3384
PpuMI 5 499, 1477, 1924, 3242, 4146
PshAI 1 1015
Psp1406I 2 6001, 6374
PstI 3 1178, 1360, 6017
PvuI 2 1034, 6143
PvuII 4 286, 361, 3933, 4008
SacI 2 580, 4227
SacII 1 151
SalI 1 2057
SapI 2 2871, 4760
ScaI 1 6254
SexAI 1 1474
SfcI 7 182, 1178, 1360, 3829, 5148
5339, 6017
SgrAI 1 3491
SmaI 4 643, 1241, 2018, 4290
SmlI 8 202, 1265, 3725, 3849, 4989
5251, 5528, 6396
SpeI 1 897
SphI 1 2723
SrfI 1 1240
SspI 1 6578
StyI 6 496, 706, 1504, 2062, 4143
4353
TatI 4 1541, 2554, 4689, 6254
TfiI 5 2293, 2520, 2716, 3426, 4858
Tsp45I 8 1282, 1491, 2565, 2626, 4538
4633, 6033, 6244
Tth111I 4 633, 1473, 4280, 4627
VspI 1 5947
XbaI 4 464, 2036, 2111, 4111
XcmI 1 2792
XhoI 1 3725
XmaI 4 643, 1241, 2018, 4290
XmnI 1 6371
Enzymes that do NOT cut molecule:
AscI BclI BlnI Bpu1102I BsaBI
BsgI BsmI BstXI Eco47III FseI
HindIII HpaI MluI NaeI NgoMIV
NruI NsiI PmeI Ppu10I RsrII
SanDI SbfI SfiI SgfI SnaBI
StuI SwaI Van91I
