pCFB载体限制性酶切位点 pCFB, 5292 bp version 013001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AatII 6 328, 381, 464, 650, 1153
5218
Acc65I 3 822, 2021, 2818
AccI 3 2142, 2232, 3178
AflII 3 202, 1440, 2373
AflIII 2 164, 3407
AgeI 1 2015
AhdI 6 862, 908, 1449, 2858, 2904
4295
Alw44I 4 1229, 3223, 3721, 4967
AlwNI 3 2494, 2569, 3818
ApaI 1 1413
ApoI 3 87, 1303, 2238
AvaI 8 785, 818, 1416, 2193, 2249
2748, 2781, 2814
BamHI 1 2244
BanII 5 939, 1413, 2751, 2764, 2935
BbeI 3 790, 1831, 2786
BbsI 1 5285
BbvCI 1 2624
BciVI 5 831, 2161, 2827, 3616, 5143
BglI 4 291, 413, 484, 4414
BglII 1 1853
BpmI 3 1974, 2321, 4385
Bpu10I 4 1723, 2508, 2583, 2624
BsaAI 4 545, 1991, 2175, 3159
BsaHI 10 328, 381, 464, 650, 790
1153, 1831, 2786, 4836, 5218
BsaI 9 869, 890, 957, 1589, 1977
2865, 2886, 2953, 4367
BsaWI 5 138, 2015, 3613, 3760, 4591
BseRI 5 893, 1243, 1736, 1775, 2889
BsiEI 8 1136, 1209, 2013, 2255, 3320
3744, 4667, 4816
BsiHKAI 6 1229, 2751, 3223, 3721, 4882
4967
BsmBI 6 1151, 1268, 1512, 1571, 1757
3056
Bsp120I 1 1413
BspHI 3 4127, 5135, 5240
BspLU11I 2 164, 3407
BsrBI 5 1474, 2252, 2277, 3338, 5139
BsrDI 2 4354, 4536
BsrGI 1 1716
BssHII 1 2734
BssSI 3 3580, 4964, 5271
Bst1107I 1 3178
BstAPI 3 2493, 2568, 3225
BstEII 1 1521
BstF5I 10 777, 857, 1561, 1748, 2206
2853, 3082, 4280, 4461, 4748
Bsu36I 1 1451
Cfr10I 2 2015, 4380
DraI 3 4164, 4183, 4875
DraIII 1 2048
DrdI 2 3096, 3509
DsaI 3 151, 567, 1106
EaeI 7 1002, 1136, 1543, 1564, 1843
2255, 4688
EarI 4 1509, 1739, 3285, 5089
Ecl136II 1 2751
Eco52I 2 1136, 2255
Eco57I 2 3934, 4982
Eco72I 1 2175
EcoNI 1 1822
EcoO109I 4 1652, 2099, 2670, 5275
EcoRI 1 2238
EcoRV 2 2479, 2554
EheI 3 790, 1831, 2786
FokI 10 777, 857, 1561, 1748, 2206
2853, 3082, 4280, 4461, 4748
FspI 1 4520
HaeII 5 790, 1831, 2786, 3281, 3651
HgaI 7 724, 1064, 1341, 3113, 3509
4087, 4837
HincII 2 2232, 4839
KasI 3 790, 1831, 2786
KpnI 3 822, 2021, 2818
MboII 10 1509, 1740, 2271, 3286, 4057
4148, 4903, 4981, 5090, 5286
MscI 3 1002, 1543, 1843
MslI 5 159, 568, 4548, 4707, 5066
MspA1I 9 151, 918, 2457, 2532, 2914
3117, 3747, 3992, 4933
MunI 2 11, 21
NarI 3 790, 1831, 2786
NcoI 1 567
NdeI 4 440, 1839, 1847, 3229
NheI 5 6, 16, 26, 197, 2368
NotI 1 2254
NsiI 1 774
NspI 4 164, 244, 3042, 3407
PleI 10 617, 812, 1195, 1220, 2038
2283, 2808, 3307, 3778, 4295
Ppu10I 1 774
PpuMI 3 1652, 2099, 2670
PshAI 1 1190
Psp1406I 2 4525, 4898
PstI 3 1353, 1535, 4541
PvuI 2 1209, 4667
PvuII 2 2457, 2532
SacI 1 2751
SacII 1 151
SalI 1 2232
SapI 1 3284
ScaI 1 4778
SchI 10 617, 812, 1195, 1220, 2038
2283, 2808, 3307, 3778, 4295
SexAI 1 1649
SfcI 7 182, 1353, 1535, 2353, 3672
3863, 4541
SmaI 4 818, 1416, 2193, 2814
SmlI 8 202, 1440, 2249, 2373, 3513
3775, 4052, 4920
SnaBI 1 545
SpeI 1 1072
SphI 1 244
SrfI 1 1415
SspI 1 5102
StyI 5 567, 881, 1679, 2667, 2877
TaqI 9 1472, 1783, 1794, 2225, 2233
2250, 2294, 3507, 4951
TatI 7 424, 504, 537, 588, 1716
3213, 4778
TfiI 1 3382
Tsp45I 6 1457, 1666, 3062, 3157, 4557
4768
TspRI 10 1459, 2050, 3303, 3809, 3822
4093, 4242, 4347, 4694, 4721
Tth111I 4 808, 1648, 2804, 3151
VspI 1 4471
XbaI 2 2211, 2635
XhoI 1 2249
XhoII 8 1853, 2244, 4048, 4059, 4145
4157, 4925, 4942
XmaI 4 818, 1416, 2193, 2814
XmnI 1 4895
Enzymes that do NOT cut molecule:
AccIII AscI BclI BlnI
Bpu1102I BsaBI BsgI BsiWI
BsmI BspMI BstBI BstXI
ClaI Eco47III FseI HindIII
HpaI MluI NaeI NgoMIV
NruI PacI PmeI RsrII
SanDI SbfI SfiI SgfI
SgrAI StuI SwaI Van91I
XcmI
