The promoter-less pEBNA-DEST expression vector is an episomal vector that permits extra-chromosomal (episomal) replication in mammalian cells. Episomal replication is facilitated by an EBV origin of replication (EBV-oriP) and a nuclear antigen, EBNA-1. Episomal replication enables transient, high level expression of a gene of interest.
Please note: the pEBNA-DEST expression vector requires the cloning of a promoter upstream of the gene of interest, which is facilitated by any of the MultiSite Gateway Pro kits.
pREP7载体是附加体型载体，可以在基因组外自主复制，类似的载体有：
pREP4 PCEP4 pREP7 pREP10 pEBNA-DEST pEBVHisA pEBVHisB pEBVHisC pREP4/CAT pEBVHis-lacZ
