The pAd/PL-DEST Gateway Vector Kit contains the Gateway-adapted ViraPower adenoviral expression vector, pAd/PL-DEST vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.
Advantages
High efficiency and rapid recombination cloning
Produces high titer adenoviral stocks
Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
Allows gene of interest to be controlled by a promoter of choice
Produces replication-incompetent virus for enhanced biosafety of the system
Amenable for use in high-throughput applications
Key Features
Gateway Technology for efficient and rapid cloning
Promoterless vector that allows gene of interest to be controlled by a promoter of choice
Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
Ampicillin selection marker
