The pLenti7.3/V5-DEST Gateway Vector Kit contains the Gateway-adapted ViraPower HiPerform lentiviral expression vector, pLenti7.3/V5-DEST for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti7.3/V5-DEST vector is equipped with two key genetic elements, making it a HiPerform vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements. In addition, the vector allows for an accurate determination of titer of functional lentivirus in just two days using Emerald Green Fluorescent Protein (EmGFP).
Advantages
Transient expression
Short-term experiments
High-throughput screening
2 day titer of functional virus using EmGFP
Flexible and versatile Gateway recombination cloning technology
Key Features
WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, allows increased transgene expression and cPPT (Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome
Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest
SV40 promoter driving expression of EmGFP
Emerald Green Fluorescent Protein (EmGFP) allows easy determination of Lentiviral titers by flow cytometry and monitor transduced cells.
