pLenti6/capTEV/CT-DEST载体关键功能与特点 The NativePure Lentiviral Expression and Affinity Purification System provides an efficient method for lentiviral delivery of your gene of interest into cells, and the purification and analysis of native protein complexes. The NativePure Lentiviral Gateway Vectors allow N- or C- terminal fusion of your recombinant protein of interest to the capTEV± affinity purification tag.
The Lentiviral Expression and Affinity Purification System provides:
Gateway vectors designed to express your protein of interest in multiple expression systems
Lentiviral vectors with the capTEV Tag that allow in vivo biotinylation of your protein of interest
Streamlined purification protocols with Streptavidin Agarose
Contents:
The NativePure Lentiviral Expression and Affinity Purification System includes the NativePure Lentiviral Gateway Vector Kit, the NativePure Lentiviral Expression Kit, and the NativePure Lentiviral Affinity Purification Kit. Each kit is also available separately.
The NativePure Lentiviral Gateway Vector Kit consists of the pLenti6/capTEV-NT-DEST1 (N-terminal vector), pLenti6/capTEV-CT-DEST (C-terminal vector), and pLenti6/capTEV-CT-GW/ARP2 (C-terminal) control vector.
The NativePure Lentiviral Expression Kit contains the NativePure Lentiviral Gateway Vector Kit, the ViraPower Bsd Lentiviral Support Kit, 293FT Cell Line, One Shot Stbl3 Chemically Competent E. coli, and Gateway LR Clonase Enzyme Mix.
The NativePure Lentiviral Affinity Purification Kit contains the NativePure Lentiviral Gateway Vector Kit, Streptavidin Agarose, NativePure 5X Lysis/Binding Buffer, NativePure 10X TEV Cleavage Buffer, 100 mM DTT (Dithiothreitol), 10% NP40, AcTEV Protease, NativePure Columns, and NativePure Concentrators.
Store the 293FT Cell Line in liquid nitrogen and the One Shot Stbl3 Chemically Competent E. coli at -80°C.
Store Streptavidin Agarose, 10% NP-40, NativePure± 5X Lysis/Binding Buffer, NativePure± 10X TEV Cleavage Buffer, Lipofectamine 2000, NativePure± Columns, and NativePure± Concentrators at +4°C.
Store the NativePure Lentiviral Gateway Vector Kit, AcTEV± Protease, 100 mM DTT, Gateway LR Clonase Enzyme Mix, ViraPower packaging Mix, and Blasticidin at -20°C. All components are guaranteed stable for 6 months when properly stored.
