The Champion pET-DEST42 Gateway destination vector is designed for rapid cloning with a Gateway entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion pET-DEST42 destination vector offers:
Bacteriophage T7lac promoter for high-level expression
O operator sequences for lac repressor binding to ensure tighter regulation of transcription
pBR322 ori for minimal basal expression
C-terminal V5 and 6xHis tags for efficient detection and purification
R sites for efficient recombination with any attL-flanked Gateway entry vector
