pCas-Guide-EF1a-CD4载体特征 All-in-one vector
Expresses human codon-optimized Cas9, C-terminal Myc-DDK tagged
BamH I and BsmB I sites are used for genomic target sequence cloning
Expresses gRNA containing the inserted target sequence and the gRNA scaffold
EF1a promoter driven CD4 to enrich transfected cells
CF3 primer (GE100008) can be used to sequence the target sequence after cloning