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pMetLuc2-Control

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  • 产品名称:pMetLuc2-Control
  • 产品型号:
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2017-08-01
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简单介绍
pMetLuc2-Control的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到pMetLuc2-Control后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
产品描述

pMetLuc2-Control载体基本信息

载体名称: pMetLuc2-Control
质粒类型: 慢病毒载体;无启动子载体;荧光素酶报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 4784 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
筛选标记: 新霉素(Neomycin)
克隆菌株: DH5alpha等
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: pMetLuc2-Control组成型表达荧光素酶MetLuc,作为pMetLuc2-Reporter载体的阳性对照;
MetLuc是已知*小分子量的荧光素酶;
荧光素酶报告基因可以用来定性定量分析启动子及其它顺式转录元件的活性。
产品目录号: 631729
稳定性: 瞬表达 或 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pMetLuc2-Control载体质粒图谱和多克隆位点信息

pMetLuc2-Control载体图谱



pMetLuc2-Control 载体特征

pMetLuc2-Control载体简介

pMetLuc2-Control载体描述 The pMetLuc2-Control vector encodes a sequence-optimized, secreted luciferase from the marine copepod Metridia longa. The 24 kDa Metridia luciferase (MetLuc) contains a 17 amino acid, N-terminal signal peptide that allows the protein to be efficiently secreted into the cell culture medium (1), making it easy to detect without cell lysis.Expression of MetLuc is driven by the constitutively active cytomegalovirus immediate early promoter (PCMV IE). SV40 polyadenylation signals downstream of the MetLuc gene direct proper processing of the 3' end of the MetLuc mRNA. The vector backbone contains an SV40 origin for replication in mammalian cells expressing the SV40 large T antigen, a pUC origin of replication for propagation in E. coli, and an f1 origin for single-stranded DNA production. A neomycin-resistance cassette (Neor) allows stably transfected eukaryotic cells to be selected using G418. This cassette consists of the SV40 early promoter, the Tn5 kanamycin/neomycin resistance gene, and polyadenylation signals from the herpes simplex virus thymidine kinase (HSV TK) gene. The vector also contains a synthetic transcription blocker (TB), composed of adjacent polyadenylation and transcription pause sites, that reduces background readthrough transcription (2). A bacterial promoter (PKanr) upstream of the cassette allows kanamycin resistance in E. coli.

The pMetLuc2-Control vector can be used as a positive control for the constitutive expression and secretion of MetLuc in experiments using the pMetLuc2-Reporter vector. pMetLuc2-Control can also be used to monitor the inhibitory effect of drugs, such as Brefeldin A, on protein secretion.
The pMetLuc2-Control Vector can be transfected into mammalian cells using any standard transfection method. Stable transfectants can be selected using G418 when required (3). The presence of MetLuc in the cell culture medium can be easily detected by adding luciferase substrate to a small aliquot of the medium and analyzing the sample with a luminometer.

Propagation in E. coli
 Suitable host strains: DH5α, HB101, and other general purpose strains. Single-stranded DNA production
requires a host containing an F plasmid such as JM109 or XL1-Blue.
 Selectable marker: plasmid confers resistance to kanamycin (50 μg/ml) in E. coli hosts.
 E. coli replication origin: pUC
 Plasmid incompatibility group: pMB1/Col E1

Note: The attached sequence file has been compiled from information in the sequence databases, published literature, and other sources, together with partial sequences obtained by Clontech. This vector has not been completely sequenced. References 1. Markova, S.V. et al. (2004) J. Bio. Chem. 279(5):3212-3217.
2. Eggermont, J. & Proudfoot, N. (1993) EMBO J. 12(6):2539–2548.
3. Gorman, C. (1985) DNA cloning: A Practical Approach, Vol. II. Ed. D. M. Glover. (IRL Press Oxford, U.K.), pp. 143-190. 

pMetLuc2-Control载体序列

hz-8145R CHCHD5  卷曲螺旋结构域蛋白CHCHD5抗体
hz-8146R CHCHD6  卷曲螺旋结构域蛋白CHCHD6抗体
hz-8148R CHCHD8  卷曲螺旋结构域蛋白CHCHD8抗体
hz-8149R Slc22a5  溶质载体家族蛋白22成员5抗体
hz-8150R NPFF/FMRFamide  **痛觉调节肽NPFF抗体
hz-8151R FAM36A  FAM36A蛋白抗体
hz-8152R FAM50A  FAM50A蛋白抗体
hz-8153R FAM134C  FAM134C蛋白抗体
hz-8154R Histone H2A.Z/ H2AFZ  组蛋白H2AZ抗体
hz-8155R GM130  高尔基体自身蛋白GM130抗体
hz-8156R FBRSL1  乙型肝炎病毒X蛋白反转录蛋白9抗体
hz-8157R GMRP1/BTBD10  糖代谢相关蛋白1抗体
hz-8158R BTBD1/HCV NS5A transactivated protein 8  丙肝病毒NS5A反转录蛋白8抗体
hz-8159R BTBD6  BTBD6蛋白抗体
hz-2282R PPO  腺样癌特异性相关抗原PPO抗体
hz-8160R G protein alpha  G蛋白α抗体
hz-8161R G protein beta subunit like  G蛋白β样蛋白抗体
hz-8162R CHD3  ATP依赖的解旋酶CHD3抗体

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