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pCMV-p53

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  • 产品名称:pCMV-p53
  • 产品型号:
  • 产品展商:HZbscience
  • 产品文档:无相关文档
  • 发布时间:2017-08-03
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简单介绍
pCMV-p53的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到pCMV-p53后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
产品描述

pCMV-p53载体基本信息

载体名称: pCMV-p53 、 p53 Dominant-Negative Vector
质粒类型: 信号通路分析载体;显性抑制载体
克隆方法: --
启动子: CMV
载体大小: 5167 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
筛选标记: Neomycin
克隆菌株: DH5a 或 HB101
宿主细胞(系): --
备注: pCMV-p53载体是信号通路分析载体;
p53是信号转导蛋白,p53的突变体是显性负性突变体,抑制信号转导过程。
产品目录号: 632420
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pCMV-p53载体质粒图谱和多克隆位点信息

pCMV-p53载体图谱



pCMV-p53载体特征

pCMV-p53载体简介

 p53 The p53 Dominant-Negative Vector Set lets you study p53 and related signaling pathways. These vectors constitutively express high levels of wild-type p53 or its dominant-negative mutant. To determine the effects of p53 expression on a particular cell line, simply transfect with the vector containing the gene for wild-type p53, and examine morphology or look for changes in the expression of other pathway proteins. 载体描述 The p53 Dominant-Negative Vector Set is a convenient tool for monitoring p53-mediated signal transduction pathways. This vector set consists of two vectors, pCMV-p53 and pCMV-p53mt135. pCMV-p53 expresses the wild-type p53 tumor suppression protein and pCMV-p53mt135 expresses a dominant-negative mutant. Both proteins are expressed at high levels from the constitutive CMV promoter. The p53 gene and p53mt135 gene differ by a G to A conversion at nucleotide 1017.
The SV40 polyadenylation sequence directs proper processing of the 3' end of the p53 or p53mt135 mRNAs. The vector backbone contains an SV40 origin for replication in mammalian cells expressing the SV40 T antigen. A neomycin-resistance cassette (Neor)—consisting of the SV40 early promoter, the Tn5 neomycin/kanamycin resistance gene, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene—allows kanamycin selection in E. coli and neomycin selection in eukaryotic cells. The vector backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production. 使用 pCMV-p53 is used to examine the physical interactions between p53 and other proteins, to induce p53-mediated cell cycle arrest, or to study p53-induced gene expression. pCMV-p53mt135 expresses the p53mt135 mutant, which because of a conformational change, can no longer interact with p53-binding sites. When p53mt135 and p53 are co-expressed, they form a mixed tetramer that is unable to interact with p53-binding sites; therefore, the downstream effects of p53 are blocked (1, 2).
Both vectors can be transfected into mammalian cells using any standard method. Stable transformants can be selected using G418 (3). Propagation in E. coli Suitable host strains: DH5a HB101 and other general purpose strains. Single-stranded DNA production requires
a host containing an F plasmid such as JM101 or XL1-Blue.
Selectable marker: plasmid confers resistance to kanamycin (50μg/ml) in E. coli hosts.
E. coli replication origin: pUC
Copy number: ~500
Plasmid incompatibility group: pMB1/ColE1 

pCMV-p53载体序列

hz-4085R phospho-c-Abl(Tyr272)  磷酸化非受体酪氨酸激酶c-Abl抗体
hz-4086R phospho-c-Abl(Tyr276)  磷酸化非受体酪氨酸激酶c-Abl��体
hz-4087R SPTLC1  丝氨酸棕榈酰转移酶1抗体
hz-4088R SLC27A5  脂肪酸转运蛋白5抗体
hz-2932R Clathrin heavy chain/Clathrin HC  网格蛋白重链抗体
hz-2933R RAB-14  RAS相关蛋白癌基因Rab14抗体
hz-2936R ARHG7  p21活化蛋白激酶ARHG7抗体
hz-2938R TdT/DNTT  末端脱氧核苷酸转移酶抗体
hz-5183R phospho-c-Abl(Thr754)  磷酸化非受体酪氨酸激酶c-Abl抗体
hz-2940R FUCA1/Alpha L fucosidase I  α-L岩藻糖苷酶抗体
hz-2941R TNFR1/TNF Receptor I  肿瘤坏死因子受体1抗体
hz-2942R HHV8/ORF K14  人类疱疹病毒8 ORF14抗体
hz-2943R ShzA6D  轴突导向因子ShzA6D抗体
hz-2944R plant lectin B4/IB4  植物凝集素IB4
hz-2945R SNX1/Sorting nexin1  分选连接蛋白1抗体
hz-2947R ERG25  甲基固醇羟化酶单加氧酶1抗体
hz-5184R phospho-c-Abl(Tyr283)  磷酸化非受体酪氨酸激酶c-Abl抗体
hz-2950R Cytosine deaminase  胞嘧啶脱氨酶抗体
hz-2951R SQSTM1/p62  泛素结合蛋白P62抗体

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