- 如果您对该产品感兴趣的话，可以
- 产品名称：AKT1 / AKT2 / AKT3 (phospho Tyr315 / Tyr316 / Tyr312) Antibody
- 产品型号：
- 产品展商：immunoclone
- 产品文档：无相关文档
- 发布时间：2017-08-10
- 在线询价
简单介绍
AKT1 / AKT2 / AKT3 (phospho Tyr315 / Tyr316 / Tyr312) Antibody迅速增长的产品线包括一抗、二抗、**测定及细胞检测试剂盒、蛋白/多肽、激动剂/拮抗剂/活化剂/抑制剂及裂解物等。AKT1 / AKT2 / AKT3 (phospho Tyr315 / Tyr316 / Tyr312) Antibody严格的质量控制和验证实验包括各种不同的应用：WB、细胞**荧光、**组化、流式细胞术、ELISA、CHIP、IP和peptide array。
产品描述
AKT1 / AKT2 / AKT3 (phospho Tyr315 / Tyr316 / Tyr312) AntibodyProduct Profile
|
Product Name
|
AKT1 / AKT2 / AKT3 (phospho Tyr315 / Tyr316 / Tyr312) Antibody
|
|
Antibody Type
|
Primary Antibodies
|
|
Product description
|
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.1) Nelms K, et al. (1999) Annu Rev Immunol. 17:701-738. Malabarba M G, et al. (1996) Biochem. J. 319:865-872. Hou J, et al. (1994) Science. 265:1701-1706. Quelle F W, et al. (1995) Mol Cell Biol. 15: 3336-3343.
More
|
|
Immunogen
|
AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y (p) -L-A) derived from Human AKT1/AKT2/AKT3.
|
AKT1 / AKT2 / AKT3 (phospho Tyr315 / Tyr316 / Tyr312) AntibodyKey Feature
|
Clonality
|
Polyclonal
|
|
Host Species
|
Rabbit
|
|
Tested Applications
|
IFIHCWB
|
|
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|
Species Reactivity
|
HumanMouseRat
|
|
Concentration
|
1mg/ml
|
|
Purification
|
Affinity purified
|
AKT1 / AKT2 / AKT3 (phospho Tyr315 / Tyr316 / Tyr312) AntibodyTarget Information
|
Gene Symbol
|
AKT1 AKT2 AKT3
|
|
Alternative Names
|
AKT
PKB
RAC
CWS6
PRKBA
PKB-ALPHA
RAC-ALPHA
PKD
PKD1
PRKCM
RAC-PK-alpha
Protein kinase B
More
|
|
Molecular Weight(MW)
|
60 kDa
|
|
Tissue Specificity
|
This antibody detects endogenous level of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312.
|
AKT1 / AKT2 / AKT3 (phospho Tyr315 / Tyr316 / Tyr312) AntibodyDatabase Links
|
Entrez Gene
|
207 / 208 / 10000
|
|
Protein Accession
|
NP_001014431.1 / NP_001617.1 / NP_005456.1
|
AKT1 / AKT2 / AKT3 (phospho Tyr315 / Tyr316 / Tyr312) AntibodyApplication
-
Application
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312).
-
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312).
|
Application Notes
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
Additional Information
|
Form
|
Liquid
|
|
Storage Instructions
|
Store antibody at -20˚C for up to one year.
|
|
Storage Buffer
|
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
|
