Histone H3.1抗体可供应相应抗体的生物素、辣根过氧化物酶、荧光素（FITC、PE、 CY3、 CY5、 CY7、 PE- CY3/5/7)标记抗体，我司主推四大类：一. 抗体（antibody）：182000种的一抗。可应用于Western blot，IP，IHC，IF，FACS，ELISA等实验。二. 蛋白(protien)：20000种的高纯度蛋白。三. elisa试剂盒(elisa kit)：可以提供超过6600种的ELISA试剂盒，包括人源，大鼠源，小鼠源等。四. 小分子(Small Molecules)：超过25000种的生物活性小分子，主要包括：拮抗剂、抑制剂、***、天然产物。
英文名称：Histone H3.1抗体
中文名称组蛋白H3.1抗体
别 名H3 histone family, member A; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member E; H3 histone family, member F; H3 histone family, member G; H3 histone family, member H; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3.1; H3.f; H3/A; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31_HUMAN; H3F1K; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1 cluster H3A; HIST1 cluster H3B; HIST1 cluster H3C; HIST1 cluster H3D; HIST1 cluster H3E; HIST1 cluster H3F; HIST1 cluster H3G; HIST1 cluster H3I; HIST1 cluster H3J; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone 1, H3b; Histone 1, H3c; Histone 1, H3e; histone 1, H3f; histone 1, H3g; histone 1, H3h; histone 1, H3i; histone 1, H3j; histone cluster 1, H3a; histone cluster 1, H3b; Histone cluster 1, H3c; Histone cluster 1, H3e; histone cluster 1, H3g; histone cluster 1, H3h; histone cluster 1, H3i; histone cluster 1, H3j; Histone gene cluster 1 H3A; Histone gene cluster 1 H3B; Histone gene cluster 1 H3E; Histone gene cluster 1 H3F; Histone gene cluster 1 H3G; Histone gene cluster 1, H3; Histone gene cluster 1, H3C; Histone gene cluster 1, H3D; Histone gene cluster 1, H3I; Histone gene cluster 1, H3IH; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
说 明 书100ul 200ul
研究领域细胞生物 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, Chimpanzee,
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量15kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Histone H3.1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Histone H3.1抗体特异性问题。从本质上来说，抗体的工作原理就是与特定蛋白或其他抗原结合，从而实现定性，定量，定位等检测。所以，抗体能否**的结合抗原，是成败的关键，抗体的特异性（specificity)就显得尤其重要了。而对特异性影响*大的，莫过于抗体的交叉反应性(cross-reactivity)。在特异性正常情况下，Histone H3.1抗体只能结合**的一种蛋白。而如果抗体本身出现问题（如结构等），可能会结合其它非目标蛋白，从而导致特异性降低。更令人恼火的是，如果抗体的特异性问题很难被检测出来。假设某个糟糕的抗体X理论上只结合蛋白A，但是它也可以结合蛋白B。当实验样本里蛋白B没有出现时，它将完全得到完全正确的实验结果。如果当样本中包含蛋白B，偏差就出现了。试想一下，你在应用抗体X时，如果你在项目前期没有使用过蛋白B样本，一切都会很顺利。但是在项目后期，你开始加入蛋白B样本，你的实验结果就完全乱了套。那么问题就来了：你事先并不知道抗体X可以结合非目标蛋白B，所以你完全无法解释为什么项目的前后会出现偏差。
Histone H3.1抗体出现交叉反应，导致特异性降低，主要是由抗体本身的质量问题引起的。由于抗体本身结构和生产过程的复杂性，生产商稍有疏忽，抗体质量就会出现问题。由于抗体是科研的刚需产品，利润又很高，所以引得众多公司疯狂进入到这个市场，直接导致了市面抗体产品质量良莠不齐，供应商鱼龙混杂。而市场本身又缺少有力的监督和管理机制，抗体频频出现质量问题也就不足为奇了。
