企业信息
第7年
- 入驻时间： 2015-11-06
- 联系人：顾磊
- 电话：021-60514606
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- Email：shybio@126.com
产品分类
产品详情
简单介绍：
β-内啡肽(βEP)检测试剂盒用于测定血清，血浆及相关液体等样本，例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、细胞培养上清、组织匀浆等本标本。产品种类齐全、质量可靠、价格优惠、灵敏度高、效果稳定、易保存、操作简单。
详情介绍：
β-内啡肽(βEP)检测试剂盒ELISA Kit for Beta-Endorphin (bEP)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | YBA806Ra |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids |
| Format | 96T |
| Assay length | 2.5 hours |
| Detection range | 12.35-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 4.62pg/mL. |
β-内啡肽(βEP)检测试剂盒Specificity
This assay has high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP).
No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.
No significant cross-reactivity or interference between Beta-Endorphin (bEP) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Beta-Endorphin (bEP) and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-Endorphin (bEP) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 80-97 | 93 |
| EDTA plasma(n=5) | 82-91 | 87 |
| heparin plasma(n=5) | 85-105 | 88 |
β-内啡肽(βEP)检测试剂盒Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
β-内啡肽(βEP)检测试剂盒Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-Endorphin (bEP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-103% | 80-92% | 86-94% | 83-101% |
| EDTA plasma(n=5) | 88-95% | 79-103% | 80-103% | 94-103% |
| heparin plasma(n=5) | 79-99% | 95-103% | 98-105% | 99-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
β-内啡肽(βEP)检测试剂盒Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1 | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Reagent Diluent | 1×300µL | Stop Solution | 1×6mL |
| TMB Substrate | 1×9mL | Instruction manual | 1 |
| Wash Buffer (30 × concentrate) | 1×20mL |
β-内啡肽(βEP)检测试剂盒Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37oC;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
β-内啡肽(βEP)检测试剂盒Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Beta-Endorphin (bEP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Beta-Endorphin (bEP) and unlabeled Beta-Endorphin (bEP) (Standards or samples) with the pre-coated antibody specific to Beta-Endorphin (bEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Beta-Endorphin (bEP) in the sample.
