企业信息
第7年
- 入驻时间： 2015-11-06
- 联系人：顾磊
- 电话：021-60514606
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- Email：shybio@126.com
产品分类
产品详情
简单介绍：
补体成分5a(C5a)检测试剂盒用于测定血清，血浆及相关液体等样本，例如适合检测包括血清、血浆、尿液、胸腹水、灌洗液、细胞培养上清、组织匀浆等本标本。产品种类齐全、质量可靠、价格优惠、灵敏度高、效果稳定、易保存、操作简单。
详情介绍：
补体成分5a(C5a)检测试剂盒CLIA Kit for Complement Component 5a (C5a)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | YBA388Ra |
| Sample type | Serum, plasma and other biological fluids |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.14-100ng/mL The standard curve concentrations used for the CLIA’s were 100ng/mL, 33.33ng/mL, 11.11ng/mL, 3.7ng/mL, 1.23ng/mL, 0.41ng/mL, 0.14ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.07ng/mL. |
补体成分5a(C5a)检测试剂盒Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Component 5a (C5a).
No significant cross-reactivity or interference between Complement Component 5a (C5a) and analogues was observed.
No significant cross-reactivity or interference between Complement Component 5a (C5a) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Complement Component 5a (C5a) and the recovery rates were calculated by comparing the measured value to the expected amount of Complement Component 5a (C5a) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 85-98 | 92 |
| EDTA plasma(n=5) | 86-93 | 89 |
| heparin plasma(n=5) | 78-93 | 83 |
补体成分5a(C5a)检测试剂盒Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 5a (C5a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 5a (C5a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 5a (C5a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Complement Component 5a (C5a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 99-105% | 87-102% | 88-96% |
| EDTA plasma(n=5) | 83-102% | 95-102% | 94-101% | 92-101% |
| heparin plasma(n=5) | 90-98% | 78-95% | 81-104% | 94-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
补体成分5a(C5a)检测试剂盒Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Substrate A | 1×10mL | Substrate B | 1×2mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
补体成分5a(C5a)检测试剂盒Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37oC;
8. Read RLU value immediately.
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37oC;
8. Read RLU value immediately.
补体成分5a(C5a)检测试剂盒Test principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 5a (C5a) level in the sample or standard.
