产品名称：A549细胞，人非小细胞肺癌细胞

A549细胞，人非小细胞肺癌细胞 Cat NO.: YB-0016 General Information: Organism: Homo sapiens, human Tissue: lung Culture Properties: adherent Morphology: epithelial Culture Method: Complete Growth Medium: RPMI-1640 (Gibco 31800-022)+10% FBS+ Penicillin/Streptomycin Subculturing: Volumes are given for a 25cm 2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. 1. Remove and discard culture medium. 2. Briefly rinse the cell layer with 0.25%(w/v) Trypsin-EDTA solution to remove all traces of serum that contains trypsin inhibitor. 3. Add 1.0 to 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. 4. Add 6.0 to 8.0mL of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. Cultures can be established between 2×103 and 1×104 viable cells/cm2 . Do not exceed 7×104 cells/cm2 . 6. Incubate cultures at 37°C, 5% CO2. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Cryopreservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase

细胞名称：A549细胞，人非小细胞肺癌细胞

组织来源：肺癌

培养条件：RPMI-1640 +10% FBS

形       态：贴壁；上皮细胞样

背       景：A549细胞，人非小细胞肺癌细胞是1972年由Giard DJ通过肺癌组织移植培养建系的，源自一位58岁的白人男性。A549能通过胞苷二磷脂酰胆碱途径合成富含不饱和脂肪酸的卵磷脂；角蛋白阳性。

A549细胞，人非小细胞肺癌细胞购买细胞注意事项：

1. 1. 收到细胞后首先观察细胞瓶是否完好，培养液是否有漏液、浑浊等现象，若有上述现象发生请及时和我们联系。
   
2. 2. 仔细阅读细胞说明书，了解细胞相关信息，如细胞形态、所用培养基、血清比例、所需细胞因子等。
   
3. 3. 用75%酒精擦拭细胞瓶表面，显微镜下观察细胞状态。因运输问题贴壁细胞会有少量从瓶壁脱落，将细胞置于培养箱内静置培养过夜，隔天再取出观察。此时多数细胞均会贴壁，若细胞仍不能贴壁请用台盼蓝染色测定细胞活力，如果证实细胞活力正常，请将细胞离心后用新鲜培养基再次贴壁培养；如果染色结果显示细胞无活力，请拍下照片及时和我们联系，信息确认后我们为您再免费寄送一次。
4. 4. 建议客户收到A549细胞，人非小细胞肺癌细胞后前3天各拍几张细胞照片，记录细胞状态，便于和技术部沟通交流。